Kutya nervus hypoglossusból kiinduló, rosszindulatú perifériás ideghüvely daganata (MPNST): Esetismertetés

Translated title of the contribution: Malignant peripheral nerve sheath tumour derived from nervus hypoglossus in a dog. Case report

Jakab Csaba, Arany Tóth Attila, Csébi Péter, A. Szász, M. Rusvai, Gálfi Péter, J. Kulka

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The authors describe a case of malignant peripheral nerve sheath tumour (MPNST) which derived from right side n. hypoglossus in a 5 year old Hungarian pointer male. The dog was anorectic in the antecedent weeks, coughed sometimes, often slavered and vomited many times. Upon physical examination medialy from the right branch of the mandible a cigar-like brief bundle with a longitude of 6-7 cm was noted. In the course of operative exploration of the fabric progeny 2 cm thick, 5 cm long real-like swelling on the right side of the n. hypoglossus was observed (Figure 1). The tumour sample was fixed in 8% neutral buffered (in PBS, pH 7.0) formalin solution for 24 hours at room temperature (Figure 2), dehydrated in a series of ethanol and xylene and embedded in paraffin. The 3-4 μm thick sections were routinely stained with hematoxylin and eosin. Fusiform or spindle cells arranged in densely or loosely sweeping fascicles, interlacing whorls, or storiform patterns were predominant. Some areas were composed of spindle or fusiform cells with high cellularity designated as Antoni A pattern, and the others comprised hypocellular abundant mucinous or myxoid matrix with occasional round cells, termed Antoni B pattern. More than four mitotic figures per high-power field were found in the tumour (Figures 3-6). Tissue of MPNST was examined immunohistochemically. For the primary antibodies, mouse monoclonal antibodies against neuron-specific enolase (NSE) (1:400; DAKO), S-100 protein (1:100; DAKO), glial fibrillary acidic protein (GFAP) (1:100; DAKO), vimentin (1:100; DAKO), cytokeratin (CK) (1:100; DAKO), desmin (1:50; DAKO), Melan-A (1:25; DAKO), claudin-5 (1:100; Zymed), Ki-67 (1:100; DAKO) and myoglobin polyclonal rabbit antibody (1:100; DAKO) were used. Immunohistochemical staining was performed using the streptavidin-peroxidase procedure: antigen-bound primary antibody was detected using standard avidin-biotin immunoperoxidase complex (DAKO LSAB2 Kit), the chromogen substrate was 3,3 - diamino-benzidine tetrahydrchloride (DAB substrate-chromogen; DAKO, Denmark) in each case. Mayer's hemalaun was used for counter-staining. The number of positive cells was calculated as follows: 10 randomly selected areas per slide were analyzed using 200x magnification with 100 cells counted in each field. The scoring standardized for each group was as follows: + + + + = 50%

Original languageHungarian
Pages (from-to)671-679
Number of pages9
JournalMagyar Allatorvosok Lapja
Volume130
Issue number11
Publication statusPublished - 2008

Fingerprint

Hypoglossal Nerve
peripheral nerves
Neurilemmoma
Dogs
case studies
neoplasms
dogs
Antibodies
Claudin-5
Claudin-1
antibodies
MART-1 Antigen
cells
Staining and Labeling
Appetite Depressants
Xylenes
Desmin
Streptavidin
Avidin
S100 Proteins

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Kutya nervus hypoglossusból kiinduló, rosszindulatú perifériás ideghüvely daganata (MPNST) : Esetismertetés. / Csaba, Jakab; Attila, Arany Tóth; Péter, Csébi; Szász, A.; Rusvai, M.; Péter, Gálfi; Kulka, J.

In: Magyar Allatorvosok Lapja, Vol. 130, No. 11, 2008, p. 671-679.

Research output: Contribution to journalArticle

@article{444e896b649248939683a4af124cd656,
title = "Kutya nervus hypoglossusb{\'o}l kiindul{\'o}, rosszindulat{\'u} perif{\'e}ri{\'a}s idegh{\"u}vely daganata (MPNST): Esetismertet{\'e}s",
abstract = "The authors describe a case of malignant peripheral nerve sheath tumour (MPNST) which derived from right side n. hypoglossus in a 5 year old Hungarian pointer male. The dog was anorectic in the antecedent weeks, coughed sometimes, often slavered and vomited many times. Upon physical examination medialy from the right branch of the mandible a cigar-like brief bundle with a longitude of 6-7 cm was noted. In the course of operative exploration of the fabric progeny 2 cm thick, 5 cm long real-like swelling on the right side of the n. hypoglossus was observed (Figure 1). The tumour sample was fixed in 8{\%} neutral buffered (in PBS, pH 7.0) formalin solution for 24 hours at room temperature (Figure 2), dehydrated in a series of ethanol and xylene and embedded in paraffin. The 3-4 μm thick sections were routinely stained with hematoxylin and eosin. Fusiform or spindle cells arranged in densely or loosely sweeping fascicles, interlacing whorls, or storiform patterns were predominant. Some areas were composed of spindle or fusiform cells with high cellularity designated as Antoni A pattern, and the others comprised hypocellular abundant mucinous or myxoid matrix with occasional round cells, termed Antoni B pattern. More than four mitotic figures per high-power field were found in the tumour (Figures 3-6). Tissue of MPNST was examined immunohistochemically. For the primary antibodies, mouse monoclonal antibodies against neuron-specific enolase (NSE) (1:400; DAKO), S-100 protein (1:100; DAKO), glial fibrillary acidic protein (GFAP) (1:100; DAKO), vimentin (1:100; DAKO), cytokeratin (CK) (1:100; DAKO), desmin (1:50; DAKO), Melan-A (1:25; DAKO), claudin-5 (1:100; Zymed), Ki-67 (1:100; DAKO) and myoglobin polyclonal rabbit antibody (1:100; DAKO) were used. Immunohistochemical staining was performed using the streptavidin-peroxidase procedure: antigen-bound primary antibody was detected using standard avidin-biotin immunoperoxidase complex (DAKO LSAB2 Kit), the chromogen substrate was 3,3 - diamino-benzidine tetrahydrchloride (DAB substrate-chromogen; DAKO, Denmark) in each case. Mayer's hemalaun was used for counter-staining. The number of positive cells was calculated as follows: 10 randomly selected areas per slide were analyzed using 200x magnification with 100 cells counted in each field. The scoring standardized for each group was as follows: + + + + = 50{\%}",
author = "Jakab Csaba and Attila, {Arany T{\'o}th} and Cs{\'e}bi P{\'e}ter and A. Sz{\'a}sz and M. Rusvai and G{\'a}lfi P{\'e}ter and J. Kulka",
year = "2008",
language = "Hungarian",
volume = "130",
pages = "671--679",
journal = "Magyar Allatorvosok Lapja",
issn = "0025-004X",
publisher = "Magyar Mezogazdasag Ltd",
number = "11",

}

TY - JOUR

T1 - Kutya nervus hypoglossusból kiinduló, rosszindulatú perifériás ideghüvely daganata (MPNST)

T2 - Esetismertetés

AU - Csaba, Jakab

AU - Attila, Arany Tóth

AU - Péter, Csébi

AU - Szász, A.

AU - Rusvai, M.

AU - Péter, Gálfi

AU - Kulka, J.

PY - 2008

Y1 - 2008

N2 - The authors describe a case of malignant peripheral nerve sheath tumour (MPNST) which derived from right side n. hypoglossus in a 5 year old Hungarian pointer male. The dog was anorectic in the antecedent weeks, coughed sometimes, often slavered and vomited many times. Upon physical examination medialy from the right branch of the mandible a cigar-like brief bundle with a longitude of 6-7 cm was noted. In the course of operative exploration of the fabric progeny 2 cm thick, 5 cm long real-like swelling on the right side of the n. hypoglossus was observed (Figure 1). The tumour sample was fixed in 8% neutral buffered (in PBS, pH 7.0) formalin solution for 24 hours at room temperature (Figure 2), dehydrated in a series of ethanol and xylene and embedded in paraffin. The 3-4 μm thick sections were routinely stained with hematoxylin and eosin. Fusiform or spindle cells arranged in densely or loosely sweeping fascicles, interlacing whorls, or storiform patterns were predominant. Some areas were composed of spindle or fusiform cells with high cellularity designated as Antoni A pattern, and the others comprised hypocellular abundant mucinous or myxoid matrix with occasional round cells, termed Antoni B pattern. More than four mitotic figures per high-power field were found in the tumour (Figures 3-6). Tissue of MPNST was examined immunohistochemically. For the primary antibodies, mouse monoclonal antibodies against neuron-specific enolase (NSE) (1:400; DAKO), S-100 protein (1:100; DAKO), glial fibrillary acidic protein (GFAP) (1:100; DAKO), vimentin (1:100; DAKO), cytokeratin (CK) (1:100; DAKO), desmin (1:50; DAKO), Melan-A (1:25; DAKO), claudin-5 (1:100; Zymed), Ki-67 (1:100; DAKO) and myoglobin polyclonal rabbit antibody (1:100; DAKO) were used. Immunohistochemical staining was performed using the streptavidin-peroxidase procedure: antigen-bound primary antibody was detected using standard avidin-biotin immunoperoxidase complex (DAKO LSAB2 Kit), the chromogen substrate was 3,3 - diamino-benzidine tetrahydrchloride (DAB substrate-chromogen; DAKO, Denmark) in each case. Mayer's hemalaun was used for counter-staining. The number of positive cells was calculated as follows: 10 randomly selected areas per slide were analyzed using 200x magnification with 100 cells counted in each field. The scoring standardized for each group was as follows: + + + + = 50%

AB - The authors describe a case of malignant peripheral nerve sheath tumour (MPNST) which derived from right side n. hypoglossus in a 5 year old Hungarian pointer male. The dog was anorectic in the antecedent weeks, coughed sometimes, often slavered and vomited many times. Upon physical examination medialy from the right branch of the mandible a cigar-like brief bundle with a longitude of 6-7 cm was noted. In the course of operative exploration of the fabric progeny 2 cm thick, 5 cm long real-like swelling on the right side of the n. hypoglossus was observed (Figure 1). The tumour sample was fixed in 8% neutral buffered (in PBS, pH 7.0) formalin solution for 24 hours at room temperature (Figure 2), dehydrated in a series of ethanol and xylene and embedded in paraffin. The 3-4 μm thick sections were routinely stained with hematoxylin and eosin. Fusiform or spindle cells arranged in densely or loosely sweeping fascicles, interlacing whorls, or storiform patterns were predominant. Some areas were composed of spindle or fusiform cells with high cellularity designated as Antoni A pattern, and the others comprised hypocellular abundant mucinous or myxoid matrix with occasional round cells, termed Antoni B pattern. More than four mitotic figures per high-power field were found in the tumour (Figures 3-6). Tissue of MPNST was examined immunohistochemically. For the primary antibodies, mouse monoclonal antibodies against neuron-specific enolase (NSE) (1:400; DAKO), S-100 protein (1:100; DAKO), glial fibrillary acidic protein (GFAP) (1:100; DAKO), vimentin (1:100; DAKO), cytokeratin (CK) (1:100; DAKO), desmin (1:50; DAKO), Melan-A (1:25; DAKO), claudin-5 (1:100; Zymed), Ki-67 (1:100; DAKO) and myoglobin polyclonal rabbit antibody (1:100; DAKO) were used. Immunohistochemical staining was performed using the streptavidin-peroxidase procedure: antigen-bound primary antibody was detected using standard avidin-biotin immunoperoxidase complex (DAKO LSAB2 Kit), the chromogen substrate was 3,3 - diamino-benzidine tetrahydrchloride (DAB substrate-chromogen; DAKO, Denmark) in each case. Mayer's hemalaun was used for counter-staining. The number of positive cells was calculated as follows: 10 randomly selected areas per slide were analyzed using 200x magnification with 100 cells counted in each field. The scoring standardized for each group was as follows: + + + + = 50%

UR - http://www.scopus.com/inward/record.url?scp=56749158164&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=56749158164&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:56749158164

VL - 130

SP - 671

EP - 679

JO - Magyar Allatorvosok Lapja

JF - Magyar Allatorvosok Lapja

SN - 0025-004X

IS - 11

ER -