Maldi-TOF MS versus 16S rRNA sequencing

Minor discrepancy between tools in identification of bacteroides isolates

Károly Péter Sárvári, J. Sóki, Miklos Iván, Cecilia Miszti, Krisztina Latkoczy, Szilviazsoka Melegh, E. Urbán

Research output: Contribution to journalArticle

Abstract

Members of the genus Bacteroides are important components of the normal microbiota of gastrointestinal tract; however, as opportunistic pathogens are also associated with severe or even life-threatening infections with significant mortality. Various species within Bacteroides fragilis group are phenotypically very similar; thus, their identifications with traditional-automated biochemical methods are frequently inaccurate. The identification of the newly discovered or reclassified bacteria can be doubtful because of the lack of biochemical profile in the database of these tests. The aim of this study was to determine the accuracy of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) method by testing of 400 Hungarian Bacteroides clinical isolates. Inaccurate identification results with MALDI-TOF MS were confirmed by 16S rRNA gene sequencing and findings were compared with traditional-automated biochemical test rapid ID 32A method as well.

Original languageEnglish
Pages (from-to)173-181
Number of pages9
JournalActa Microbiologica et Immunologica Hungarica
Volume65
Issue number2
DOIs
Publication statusPublished - Jun 1 2018

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Bacteroides
Mass Spectrometry
Lasers
Bacteroides fragilis
Microbiota
rRNA Genes
Gastrointestinal Tract
Databases
Bacteria
Mortality
Infection

Keywords

  • Bacteroides fragilis group
  • Rapid ID 32A
  • Sequencing

ASJC Scopus subject areas

  • Immunology and Microbiology(all)

Cite this

Maldi-TOF MS versus 16S rRNA sequencing : Minor discrepancy between tools in identification of bacteroides isolates. / Sárvári, Károly Péter; Sóki, J.; Iván, Miklos; Miszti, Cecilia; Latkoczy, Krisztina; Melegh, Szilviazsoka; Urbán, E.

In: Acta Microbiologica et Immunologica Hungarica, Vol. 65, No. 2, 01.06.2018, p. 173-181.

Research output: Contribution to journalArticle

Sárvári, Károly Péter ; Sóki, J. ; Iván, Miklos ; Miszti, Cecilia ; Latkoczy, Krisztina ; Melegh, Szilviazsoka ; Urbán, E. / Maldi-TOF MS versus 16S rRNA sequencing : Minor discrepancy between tools in identification of bacteroides isolates. In: Acta Microbiologica et Immunologica Hungarica. 2018 ; Vol. 65, No. 2. pp. 173-181.
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