MALDI-TOF mass spectrometry of a combinatorial peptide library: Effect of matrix composition on signal suppression

G. Schlosser, Gabriella Pocsfalvi, Emoke Huszár, Antonio Malorni, F. Hudecz

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The effect of matrix composition on signal suppression caused by a dominant compound under MALDI ionization was studied using the combinatorial TQTXT pentapeptide library as a model system. The peptide library is composed of 19 components with all proteinogenic amino acids except cysteine in position X. From these compounds, only the Arg peptide (TQTRT) was detected with sufficient intensity in the MALDI-TOF mass spectrum under typical MALDI conditions (CCA matrix). The analysis of a set of compounds utilized as different matrix components, additives and a cationizing agent revealed that the composition of the matrix is a critical point in signal suppression. Highly improved ion yields were achieved by using a CCA/DHB mixture as a matrix. The addition of K + as a cationizing agent to the CCA matrix resulted in MALDI-TOF mass spectra with relative ion intensities very similar to those obtained by electrospray ionization.

Original languageEnglish
Pages (from-to)1590-1594
Number of pages5
JournalJournal of Mass Spectrometry
Volume40
Issue number12
DOIs
Publication statusPublished - Dec 2005

Fingerprint

Peptide Library
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Peptides
peptides
Mass spectrometry
Mass Spectrometry
mass spectroscopy
retarding
Ions
Electrospray ionization
matrices
Chemical analysis
Ionization
Cysteine
Amino acids
Amino Acids
mass spectra
Libraries
ionization
cysteine

Keywords

  • Combinatorial chemistry
  • MALDI
  • Mass spectrometry
  • Peptide library
  • Signal suppression

ASJC Scopus subject areas

  • Organic Chemistry
  • Spectroscopy
  • Biophysics

Cite this

MALDI-TOF mass spectrometry of a combinatorial peptide library : Effect of matrix composition on signal suppression. / Schlosser, G.; Pocsfalvi, Gabriella; Huszár, Emoke; Malorni, Antonio; Hudecz, F.

In: Journal of Mass Spectrometry, Vol. 40, No. 12, 12.2005, p. 1590-1594.

Research output: Contribution to journalArticle

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