Malachite green treatment of industrial Penicillium chrysogenum protoplasts results in increased penicillin-V formation

B. Ribár, A. Szentirmai

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We attempted protoplast fusion in order to generate gene transfer between an industrial strain of Penicillium chrysogenum and a fission yeast, Schizosaccharomyces pombe. The Penicillium strain was treated with malachite green. The S. pombe strain was auxotrophic for lysine. The regenerated colonies showed Penicillium morphology. The number of Penicillium colonies was significantly higher when the inactivated Penicillium protoplasts were fused to S. pombe protoplasts than in the self-fusion control experiments. We randomly isolated colonies from the regeneration plates and measured beta-lactam formation in cultures from shaken flasks. Antibiotic production was increased in colonies originated from the malachite green-treated protoplasts.

Original languageEnglish
Pages (from-to)311-314
Number of pages4
JournalJournal of Industrial Microbiology and Biotechnology
Volume21
Issue number6
Publication statusPublished - 1998

Fingerprint

Penicillin V
Penicillium chrysogenum
Schizosaccharomyces
Protoplasts
Penicillium
Fusion reactions
Gene transfer
beta-Lactams
Antibiotics
Yeast
Lysine
Anti-Bacterial Agents
Regeneration
Experiments
malachite green
Genes
Lactams

Keywords

  • Beta-lactam production
  • Malachite green
  • P. chrysogenum
  • Protoplasts
  • S. pombe

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

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abstract = "We attempted protoplast fusion in order to generate gene transfer between an industrial strain of Penicillium chrysogenum and a fission yeast, Schizosaccharomyces pombe. The Penicillium strain was treated with malachite green. The S. pombe strain was auxotrophic for lysine. The regenerated colonies showed Penicillium morphology. The number of Penicillium colonies was significantly higher when the inactivated Penicillium protoplasts were fused to S. pombe protoplasts than in the self-fusion control experiments. We randomly isolated colonies from the regeneration plates and measured beta-lactam formation in cultures from shaken flasks. Antibiotic production was increased in colonies originated from the malachite green-treated protoplasts.",
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