Major distinctions in the antioxidant responses in liver and kidney of Cd2 +-treated common carp (Cyprinus carpio)

Krisztina Dugmonits, Ágnes Ferencz, Zsanett Jancsó, Renáta Juhász, E. Hermesz

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

This study is related to the accumulation of Cd2 +, its effects on oxidative stress biomarkers and its role in macromolecule damage in liver and kidney of common carp. We present evidence of an increased ratio of reduced to oxidized glutathione (GSH/GSSG) in both organs after 10 mg/L Cd2 + exposure, with different underlying biological mechanisms and consequences. In the liver, the expressions and/or activities of superoxide dismutase, catalase, glutathione reductase and glutathione peroxidase increased to cope with the Cd2 +-generated toxic effects during the first 48 h of treatment. In contrast, none of these selected antioxidant markers was significantly altered in the kidney, whereas the expression of glutathione synthetase was upregulated. These results suggest that the major defense mechanism provoked by Cd 2 + exposure involves the regeneration of GSH in the liver, while its de novo synthesis predominates in the kidney. High levels of accumulation of Cd2 + and peroxynitrite anion (ONOO-) were detected in the kidney; the major consequences of ONOO- toxicity were enhanced lipid peroxidation and GSH depletion. The accumulation of ONOO- in the kidney suggests intensive production of NO and the development of nitrosative stress. In the liver the level of hydrogen peroxide was elevated.

Original languageEnglish
Pages (from-to)225-230
Number of pages6
JournalComparative Biochemistry and Physiology - C Toxicology and Pharmacology
Volume158
Issue number4
DOIs
Publication statusPublished - 2013

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Carps
Liver
Antioxidants
Kidney
Glutathione Disulfide
Glutathione Synthase
Peroxynitrous Acid
Oxidative stress
Glutathione Reductase
Poisons
Biomarkers
Glutathione Peroxidase
Macromolecules
Catalase
Hydrogen Peroxide
Superoxide Dismutase
Anions
Toxicity
Lipid Peroxidation
Regeneration

Keywords

  • Antioxidant
  • Cadmium
  • Glutathione
  • Nitrosative stress
  • Peroxynitrite

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Physiology
  • Health, Toxicology and Mutagenesis
  • Toxicology

Cite this

Major distinctions in the antioxidant responses in liver and kidney of Cd2 +-treated common carp (Cyprinus carpio). / Dugmonits, Krisztina; Ferencz, Ágnes; Jancsó, Zsanett; Juhász, Renáta; Hermesz, E.

In: Comparative Biochemistry and Physiology - C Toxicology and Pharmacology, Vol. 158, No. 4, 2013, p. 225-230.

Research output: Contribution to journalArticle

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