Maintenance of luminal NADPH in the endoplasmic reticulum promotes the survival of human neutrophil granulocytes

T. Kardon, Silvia Senesi, Paola Marcolongo, Balázs Legeza, G. Bánhegyi, J. Mandl, Rosella Fulceri, Angelo Benedetti

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The present study demonstrates the expression of hexose-6-phosphate dehydrogenase and 11β-hydroxysteroid dehydrogenase type 1 in human neutrophils, and the presence and activity of these enzymes in the microsomal fraction of the cells. Their concerted action together with the previously described glucose-6-phosphate transporter is responsible for cortisone-cortisol interconversion detected in human neutrophils. Furthermore, the results suggest that luminal NADPH generation by the cortisol dehydrogenase activity of 11β-hydroxysteroid dehydrogenase type 1 prevents neutrophil apoptosis provoked by the inhibition of the glucose-6-phosphate transporter. In conclusion, the maintenance of the luminal NADPH pool is an important antiapoptotic factor in neutrophil granulocytes.

Original languageEnglish
Pages (from-to)1809-1815
Number of pages7
JournalFEBS Letters
Volume582
Issue number13
DOIs
Publication statusPublished - Jun 11 2008

Fingerprint

11-beta-Hydroxysteroid Dehydrogenases
NADP
Granulocytes
Endoplasmic Reticulum
Hydrocortisone
Neutrophils
Maintenance
Cortisone
Oxidoreductases
Apoptosis
Enzymes
glucose 6-phosphate(transporter)
galactose-6-phosphate dehydrogenase

Keywords

  • 11β-Hydroxysteroid dehydrogenase type 1
  • Apoptosis
  • Glucocorticoid
  • Glucose-6-phosphate transporter
  • Hexose-6-phosphate dehydrogenase

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Maintenance of luminal NADPH in the endoplasmic reticulum promotes the survival of human neutrophil granulocytes. / Kardon, T.; Senesi, Silvia; Marcolongo, Paola; Legeza, Balázs; Bánhegyi, G.; Mandl, J.; Fulceri, Rosella; Benedetti, Angelo.

In: FEBS Letters, Vol. 582, No. 13, 11.06.2008, p. 1809-1815.

Research output: Contribution to journalArticle

Kardon, T. ; Senesi, Silvia ; Marcolongo, Paola ; Legeza, Balázs ; Bánhegyi, G. ; Mandl, J. ; Fulceri, Rosella ; Benedetti, Angelo. / Maintenance of luminal NADPH in the endoplasmic reticulum promotes the survival of human neutrophil granulocytes. In: FEBS Letters. 2008 ; Vol. 582, No. 13. pp. 1809-1815.
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AU - Mandl, J.

AU - Fulceri, Rosella

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