Maintenance of human hyperplastic prostate implants at different sites in athymic mice

G. Soós, M. Debiec-Rychter, R. F. Jones, K. Zukowski, G. P. Haas, C. Y. Wang

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The present study determined the influence of implantation sites, androgens, and the graft's fibrovascular elements on the maintenance of epithelial elements of human benign hyperplastic prostate tissue (BPH) in the nude mouse. BPH fragments prepared from fresh surgical specimens were implanted subcutaneously (s.c.), intraperitoneally (i.p.), or under the renal capsules (r.c.) into male Beige nude mice, which had been implanted s.c. with a Silastic tube filled with 4-dihydrotestosterone (DHT) or cholesterol. Two weeks later the BPH tissues were removed from the mouse and examined microscopically. The implants from all three sites maintained a comparable morphology, with epithelial and/or angio-leiomyo-matous stromal hyperplastic appearance, without striking signs of atrophy, irrespective of supplementation with DHT. Expression of proliferating cell nuclear antigen in the implants was comparable, indicating that there was no significant influence of implantation site on the proliferative ability of either epithelia or the stromal fibroblasts. The PCNA-positive cells in the implants, including the vascular and myofibrous elements, hybridized in situ to a human-specific repeated-sequence DNA probe, indicating that these proliferating cells were of human origin. Our data suggest that during the early phases of the adaptation and maintenance of BPH implants, survival of epithelial cells is actively supported by fibro-vascular mesenchymal elements of the prostate grafts in a manner apparently unaffected by DHT supplements

Original languageEnglish
Pages (from-to)2007-2011
Number of pages5
JournalAnticancer Research
Volume15
Issue number5 B
Publication statusPublished - 1995

Fingerprint

Dihydrotestosterone
Nude Mice
Prostate
Proliferating Cell Nuclear Antigen
Blood Vessels
Maintenance
Transplants
DNA Probes
Androgens
Atrophy
Capsules
Epithelium
Fibroblasts
Epithelial Cells
Cholesterol
Kidney

Keywords

  • Athymic mice
  • Human
  • Prostate implants

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Soós, G., Debiec-Rychter, M., Jones, R. F., Zukowski, K., Haas, G. P., & Wang, C. Y. (1995). Maintenance of human hyperplastic prostate implants at different sites in athymic mice. Anticancer Research, 15(5 B), 2007-2011.

Maintenance of human hyperplastic prostate implants at different sites in athymic mice. / Soós, G.; Debiec-Rychter, M.; Jones, R. F.; Zukowski, K.; Haas, G. P.; Wang, C. Y.

In: Anticancer Research, Vol. 15, No. 5 B, 1995, p. 2007-2011.

Research output: Contribution to journalArticle

Soós, G, Debiec-Rychter, M, Jones, RF, Zukowski, K, Haas, GP & Wang, CY 1995, 'Maintenance of human hyperplastic prostate implants at different sites in athymic mice', Anticancer Research, vol. 15, no. 5 B, pp. 2007-2011.
Soós G, Debiec-Rychter M, Jones RF, Zukowski K, Haas GP, Wang CY. Maintenance of human hyperplastic prostate implants at different sites in athymic mice. Anticancer Research. 1995;15(5 B):2007-2011.
Soós, G. ; Debiec-Rychter, M. ; Jones, R. F. ; Zukowski, K. ; Haas, G. P. ; Wang, C. Y. / Maintenance of human hyperplastic prostate implants at different sites in athymic mice. In: Anticancer Research. 1995 ; Vol. 15, No. 5 B. pp. 2007-2011.
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