Magnetite-Binding Flagellar Filaments Displaying the MamI Loop Motif

Éva Bereczk-Tompa, M. Pósfai, Balázs Tóth, F. Vonderviszt

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

This work aimed at developing a novel method for fabricating 1 D magnetite nanostructures with the help of mutated flagellar filaments. We constructed four different flagellin mutants displaying magnetite-binding motifs: two contained fragments of magnetosome-associated proteins from magnetotactic bacteria (MamI and Mms6), and synthetic sequences were used for the other two. A magnetic selection method identified the MamI mutant as having the highest binding affinity to magnetite. Filaments built from MamI loop-containing flagellin subunits were used as templates to form chains of magnetite nanoparticles along the filament by capturing them from suspension. Our study represents a proof-of-concept that flagellar filaments can be engineered to facilitate formation of 1 D magnetite nanostructures under ambient conditions. In addition, it proves the interaction between MamI and magnetite, with implications for the role of this protein in magnetotactic bacteria.

Original languageEnglish
Pages (from-to)2075-2082
Number of pages8
JournalChemBioChem
Volume17
Issue number21
DOIs
Publication statusPublished - Nov 3 2016

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Keywords

  • biomimetic synthesis
  • flagellar filament
  • magnetic nanofiber
  • MamI protein
  • template synthesis

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Organic Chemistry

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