Macrophage-Bound C3 Fragments as Adhesion Molecules Modulate Presentation of Exogenous Antigens

Anna Erdei, Vera Köhler, Hubert Schäfer, Reinhard Burger

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The involvement of complement in the response to T cell dependent antigens is generally accepted, however the mechanism has not been clarified. We compared the T cell response in vitro, using antigen-pulsed macrophages from normal and genetically C3-deficient guinea pigs, and show, that C3-fragments fixed covalently to the surface of the antigen-presenting cells are involved in the triggering of responder T cells. Binding of guinea pig C3-specific mAb to oilelicited, OVA- and PPD-pulsed macrophages of C3D guinea pigs is reduced compared to normal cells, while the expression of Ia antigens is the same. C3-like peptides can be immunoprecipitated only from the lysate of oil-elicited normal cells. These C3-fragments are fixed to the cell-membrane via ester-bonds, since they are released upon treatment with hydroxylamine. In comparison with normal cells, the antigen-presenting capacity of macrophages derived from C3D animals is strongly impaired in cultures containing 10% normal guinea pig serum. A further impairment is observed in cultures with 10% C3D guinea pig serum. Two of the tested C3-specific mAb inhibited antigen-induced T cell proliferation in a dose-dependent manner. Our data point to the importance of C3, as a bivalent molecule, having the capacity to facilitate the cooperation between the antigen-presenting cell and the responder T lymphocytes.

Original languageEnglish
Pages (from-to)314-326
Number of pages13
JournalImmunobiology
Volume185
Issue number2-4
DOIs
Publication statusPublished - 1992

Keywords

  • BSA
  • C3 deficient
  • CJD
  • CR1, CR2 and CR3
  • FACS
  • FITC
  • LNL
  • OVA
  • PBS
  • PEC
  • PPD
  • SDS-PAGE
  • bovine serum albumine
  • complement receptors type 1, type 2 and type 3
  • fluorescein isothyocyanate
  • fluorescein-activated cell sorter
  • lymph node lymphocytes
  • mAb
  • monoclonal antibodie(s)
  • ovalbumine
  • peritoneal exudate cell(s)
  • phosphate-buffered saline
  • purified protein derivative
  • sodium dodecylsulfatepolyacrylamide gelelectrophoresis

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Hematology

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