Macrocephaly in bull spermatozoa is associated with nuclear vacuoles, diploidy and alteration of chromatin condensation

T. Revay, S. Nagy, C. Kopp, A. Flyckt, W. Rens, D. Rath, A. Hidas, A. Kovács, A. Johannisson, H. Rodriguez-Martinez, M. Andersson

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Spermatozoa from 2 dairy AI (artificial insemination) bulls (A and B), identified by their abnormal spermiogram with cells depicting frequent macrocephaly, double tails and nuclear vacuoles, were case-investigated and compared to normal spermatozoa from a control AI sire (C). Head sizes were measured and morphological abnormalities scored using brightfield and differential interference contrast microscopy. The degree of sperm maturation and of resistance to acid-induced DNA denaturation in situ were determined after uploading of acridine orange using flow cytometry of 5,000 cells/sample. Nuclear fragmentation, i.e. the ratio of red to total (red + green) fluorescence, reached 7.1% and 31% in bulls A and B, compared to 2% in bull C. The proportion of immature spermatozoa, i.e. those with incomplete histone-protamine exchange and depicting higher green fluorescence compared to the main population of the control bull, reached 9.54% in A and 7.75% in B, compared to only 0.47% in the control. In the second part of this study the previously unknown chromosomal constitution of large-headed spermatozoa of bull A was investigated by fluorescence in situ hybridization using an X-Y painting probe set. The 7.5% XY-bearing cells and the presence of diploid spermatozoa detected by flow cytometry indicate a meiotic arrest in the first division in bull A, becoming the first proven case of association of macrocephaly and M1 diploidy. The diverse approaches used for the investigation of spermatozoal DNA provide insights into the etiology of macrocephaly.

Original languageEnglish
Pages (from-to)202-209
Number of pages8
JournalCytogenetic and Genome Research
Volume126
Issue number1-2
DOIs
Publication statusPublished - Jan 2009

Fingerprint

Megalencephaly
Vacuoles
Diploidy
Chromatin
Spermatozoa
Artificial Insemination
Flow Cytometry
Fluorescence
Interference Microscopy
Sperm Maturation
Nucleic Acid Denaturation
Paintings
Acridine Orange
Protamines
Population Control
Constitution and Bylaws
Fluorescence In Situ Hybridization
Histones
Head
Acids

Keywords

  • Bull
  • Chromatin condensation
  • Diploidy
  • FISH
  • Macrocephaly
  • Nuclear vacuoles
  • Sperm

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)
  • Molecular Biology

Cite this

Macrocephaly in bull spermatozoa is associated with nuclear vacuoles, diploidy and alteration of chromatin condensation. / Revay, T.; Nagy, S.; Kopp, C.; Flyckt, A.; Rens, W.; Rath, D.; Hidas, A.; Kovács, A.; Johannisson, A.; Rodriguez-Martinez, H.; Andersson, M.

In: Cytogenetic and Genome Research, Vol. 126, No. 1-2, 01.2009, p. 202-209.

Research output: Contribution to journalArticle

Revay, T, Nagy, S, Kopp, C, Flyckt, A, Rens, W, Rath, D, Hidas, A, Kovács, A, Johannisson, A, Rodriguez-Martinez, H & Andersson, M 2009, 'Macrocephaly in bull spermatozoa is associated with nuclear vacuoles, diploidy and alteration of chromatin condensation', Cytogenetic and Genome Research, vol. 126, no. 1-2, pp. 202-209. https://doi.org/10.1159/000245921
Revay, T. ; Nagy, S. ; Kopp, C. ; Flyckt, A. ; Rens, W. ; Rath, D. ; Hidas, A. ; Kovács, A. ; Johannisson, A. ; Rodriguez-Martinez, H. ; Andersson, M. / Macrocephaly in bull spermatozoa is associated with nuclear vacuoles, diploidy and alteration of chromatin condensation. In: Cytogenetic and Genome Research. 2009 ; Vol. 126, No. 1-2. pp. 202-209.
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AU - Rath, D.

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