Lysosomal enzyme activities in frozen, non-cultured chorionic villi for prenatal diagnosis of enzymopathies.

M. Németh, A. László, A. Kovács, G. Falkay

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Normal reference values of lysosomal enzyme activities (alpha-glucosidase, mannosidase, fucosidase and arylsulfatase-A) were determined in chorionic villi obtained from artificial abortion in the first trimester of normal pregnancies (gestational weeks 6 to 11). Villi were homogenized comparatively either in saline or in Triton X-100 detergent. The alpha-glucosidase, mannosidase and arylsulfatase-A enzyme activities significantly diminished if homogenization was done in saline instead of Triton-X while the difference in fucosidase activity was not significant. Significant correlation was detected between alpha-glucosidase activity and week of gestation. It is suggested that Triton X-100-homogenization should be used for the lysosomal enzyme determinations in chorionic villi because the solubilization of enzymes from the lysosomes is complete in this case than with homogenization in saline.

Original languageEnglish
Pages (from-to)143-148
Number of pages6
JournalActa Medica Hungarica
Volume49
Issue number1-2
Publication statusPublished - 1992

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Chorionic Villi
Prenatal Diagnosis
alpha-Glucosidases
Cerebroside-Sulfatase
alpha-L-Fucosidase
alpha-Mannosidase
Octoxynol
Enzymes
Reference Values
First Pregnancy Trimester
Lysosomes
Detergents
Pregnancy

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Lysosomal enzyme activities in frozen, non-cultured chorionic villi for prenatal diagnosis of enzymopathies. / Németh, M.; László, A.; Kovács, A.; Falkay, G.

In: Acta Medica Hungarica, Vol. 49, No. 1-2, 1992, p. 143-148.

Research output: Contribution to journalArticle

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