Lysed cell removal promotes frozen-thawed embryo development

Thomas A. Elliott, Luiz Fernando A Colturato, Tyl H. Taylor, Graham Wright, Hilton I. Kort, P. Nagy

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Objective: To develop a mouse model to investigate the possible causes for increased success rates when lysed cells are removed from thawed embryos. Design: Experimental study. Setting: Clinical IVF laboratory. Intervention(s): Assisted hatching, cell lysis, and removal of lysed cells. Main Outcome Measure(s): Embryonic growth rate and morphology. Result(s): The mouse embryos were divided into three groups; control (no cell lysis), group 1 (cell lysis and removal), and group 2 (cell lysis only). There was no significant difference in the initial number of blastomeres in each group or the number of cells lysed artificially in groups 1 and 2. The rate of embryonic development showed a significant delay in group 2 (7.97 ± 4.92; control, 10.42 ± 8.18; group 1, 5.74 ±4.42; group 2). The embryo morphology on day 4 was significantly improved in group 1 and the control group when compared with group 2. Conclusion(s): Mouse embryos with artificially lysed cells after thawing had poorer developmental quality and growth rates compared with control embryos. However, removal of lysed cells restored the embryo's developmental potential to that of the control. Cell number and morphology was also significantly improved compared with embryos without lysed cell removal. These findings are consistent with human embryo development after thawing when lysed cells are present and thus mechanical lysis seems to be an appropriate method by which to further study frozen-thawed lysed cell removal.

Original languageEnglish
Pages (from-to)1444-1449
Number of pages6
JournalFertility and Sterility
Volume87
Issue number6
DOIs
Publication statusPublished - Jun 2007

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Embryonic Development
Embryonic Structures
Cell Count
Blastomeres
Human Development
Growth
Research Design
Outcome Assessment (Health Care)
Control Groups

Keywords

  • Blastocyst
  • cryopreservation
  • embryo
  • IVF
  • lysed cell removal
  • thaw

ASJC Scopus subject areas

  • Obstetrics and Gynaecology

Cite this

Elliott, T. A., Colturato, L. F. A., Taylor, T. H., Wright, G., Kort, H. I., & Nagy, P. (2007). Lysed cell removal promotes frozen-thawed embryo development. Fertility and Sterility, 87(6), 1444-1449. https://doi.org/10.1016/j.fertnstert.2006.11.064

Lysed cell removal promotes frozen-thawed embryo development. / Elliott, Thomas A.; Colturato, Luiz Fernando A; Taylor, Tyl H.; Wright, Graham; Kort, Hilton I.; Nagy, P.

In: Fertility and Sterility, Vol. 87, No. 6, 06.2007, p. 1444-1449.

Research output: Contribution to journalArticle

Elliott, TA, Colturato, LFA, Taylor, TH, Wright, G, Kort, HI & Nagy, P 2007, 'Lysed cell removal promotes frozen-thawed embryo development', Fertility and Sterility, vol. 87, no. 6, pp. 1444-1449. https://doi.org/10.1016/j.fertnstert.2006.11.064
Elliott, Thomas A. ; Colturato, Luiz Fernando A ; Taylor, Tyl H. ; Wright, Graham ; Kort, Hilton I. ; Nagy, P. / Lysed cell removal promotes frozen-thawed embryo development. In: Fertility and Sterility. 2007 ; Vol. 87, No. 6. pp. 1444-1449.
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