Lymphocytes possess an electrogenic H+-transporting pathway in their plasma membrane

K. Káldi, K. Szaszi, K. Susztak, A. Kapus, E. Ligeti

Research output: Contribution to journalArticle

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Abstract

The existence of an electrogenic H+-transporting pathway similar to that described in the plasma membrane of granulocytes and macrophages is reported in pig peripheral lymphocytes. The function of the H+-transport pathway can only be detected when free movement of charge-compensating cations is allowed. H+ transport is stimulated by arachidonic acid and various unsaturated fatty acids, and inhibited by bivalent cations, with the following sequence of efficiency: Zn2+ > Cd2+ = Co2+ = Ni2+ > Mn2+ > Ba2+ = Ca2+ = Mg2+. The transport pathway is activated by intracellular acidification and by NN'-dicyclohexylcarbodiimide, but it is not influenced by phorbol 12-myristate 13-acetate. As pig peripheral lymphocytes are not able to produce O2-, it is suggested that the operation of the electrogenic H+ conductance does not require the assembly of a functional NADPH oxidase.

Original languageEnglish
Pages (from-to)329-334
Number of pages6
JournalBiochemical Journal
Volume301
Issue number2
Publication statusPublished - 1994

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Lymphocytes
Cell membranes
Cations
Swine
Cell Membrane
Dicyclohexylcarbodiimide
Acidification
Macrophages
NADPH Oxidase
Unsaturated Fatty Acids
Granulocytes
Arachidonic Acid
Acetates
phorbol-12-myristate

ASJC Scopus subject areas

  • Biochemistry

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Lymphocytes possess an electrogenic H+-transporting pathway in their plasma membrane. / Káldi, K.; Szaszi, K.; Susztak, K.; Kapus, A.; Ligeti, E.

In: Biochemical Journal, Vol. 301, No. 2, 1994, p. 329-334.

Research output: Contribution to journalArticle

Káldi, K. ; Szaszi, K. ; Susztak, K. ; Kapus, A. ; Ligeti, E. / Lymphocytes possess an electrogenic H+-transporting pathway in their plasma membrane. In: Biochemical Journal. 1994 ; Vol. 301, No. 2. pp. 329-334.
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