Lymphocytes from rheumatoid arthritis patients have elevated levels of intracellular peroxiredoxin 2, and a greater frequency of cells with exofacial peroxiredoxin 2, compared with healthy human lymphocytes

Katalin É Szabó-Taylor, Paul Eggleton, Carly A L Turner, M. Letizia Lo Faro, Joanna M. Tarr, S. Tóth, Matt Whiteman, Richard C. Haigh, Jennifer A. Littlechild, Paul G. Winyard

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Peroxiredoxin 2 has immune regulatory functions, but its expression in human peripheral blood lymphocytes and levels in extracellular fluid in healthy subjects and rheumatoid arthritis patients are poorly described. In the present study, the median intracellular peroxiredoxin 2 protein content of lymphocytes from rheumatoid arthritis patients was more than two-fold higher compared with healthy subjects' lymphocytes. Intracellular peroxiredoxin 3 levels were similar in healthy and rheumatoid arthritis lymphocytes. Flow cytometry detected peroxiredoxin 2 on the surface of ca. 8% of T cells and ca. 56% of B cells (median % values) of all subjects analyzed. Exofacial thioredoxin-1 was also observed. In the total lymphocyte population from rheumatoid arthritis patients, few cells (median, 6%) displayed surface peroxiredoxin 2. In contrast, a significantly increased proportion of interleukin-17 +ve lymphocytes were exofacially peroxiredoxin 2 +ve (median, 39%). Prdx2 was also detected in human extracellular fluids. We suggest that crucial inflammatory cell subsets, i.e. interleukin-17 +ve T cells, exhibit increased exofacial redox-regulating enzymes and that peroxiredoxin 2 may be involved in the persistence of pro-inflammatory cells in chronic inflammation.

Original languageEnglish
Pages (from-to)1223-1231
Number of pages9
JournalInternational Journal of Biochemistry and Cell Biology
Volume44
Issue number8
DOIs
Publication statusPublished - Aug 2012

Fingerprint

Peroxiredoxins
Lymphocytes
Rheumatoid Arthritis
T-cells
Interleukin-17
Extracellular Fluid
Peroxiredoxin III
Healthy Volunteers
T-Lymphocytes
Thioredoxins
Fluids
Flow cytometry
Oxidation-Reduction
Flow Cytometry
Blood
B-Lymphocytes
Cells
Inflammation
Enzymes
Population

Keywords

  • Autoimmune disease
  • Cell surface
  • Inflammation
  • Interleukin 17
  • Peroxiredoxin
  • Thioredoxin

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology

Cite this

Lymphocytes from rheumatoid arthritis patients have elevated levels of intracellular peroxiredoxin 2, and a greater frequency of cells with exofacial peroxiredoxin 2, compared with healthy human lymphocytes. / Szabó-Taylor, Katalin É; Eggleton, Paul; Turner, Carly A L; Lo Faro, M. Letizia; Tarr, Joanna M.; Tóth, S.; Whiteman, Matt; Haigh, Richard C.; Littlechild, Jennifer A.; Winyard, Paul G.

In: International Journal of Biochemistry and Cell Biology, Vol. 44, No. 8, 08.2012, p. 1223-1231.

Research output: Contribution to journalArticle

Szabó-Taylor, Katalin É ; Eggleton, Paul ; Turner, Carly A L ; Lo Faro, M. Letizia ; Tarr, Joanna M. ; Tóth, S. ; Whiteman, Matt ; Haigh, Richard C. ; Littlechild, Jennifer A. ; Winyard, Paul G. / Lymphocytes from rheumatoid arthritis patients have elevated levels of intracellular peroxiredoxin 2, and a greater frequency of cells with exofacial peroxiredoxin 2, compared with healthy human lymphocytes. In: International Journal of Biochemistry and Cell Biology. 2012 ; Vol. 44, No. 8. pp. 1223-1231.
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