Lutein-chlorophyll-a energy transfer in detergent micelles

J. Szabad, E. Lehoczki, L. Szalay, K. Csatorday

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Abstract

Absorption, fluorescence and fluorescence excitation spectra were determined for equimolar mixed micellar detergent solutions of lutein and chlorophyll-a in the concentration range from 9·10-6 to 1.8·10-4 M, with detergent (triton-X100) concentrations from 3·-10-4 to 7·10-3 M. In the range of detergent concentrations studied the pigments incorporated into the detergent micelles attained a high local concentration (0.1 to 0.01 M), reminiscent of pigment concentration within the chloroplast. A lutein → chlorophyll-a energy transfer with an efficiency of about 15% was found in these systems. In dilute (9·10-6 M) pigment solution with concentrated (7·10-3 M) detergent practically no transfer is observed. The extent of aggregation and the efficiency of transfer depend on the composition of the system. The aggregation of chlorophyll-a is partly inhibited by lutein molecules. It is shown that the energy transfer efficiency as function of distance follows an r-3 relationship, R0 being 22 å.

Original languageEnglish
Pages (from-to)65-74
Number of pages10
JournalBiophysics of Structure and Mechanism
Volume1
Issue number1
DOIs
Publication statusPublished - Mar 1 1974

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Keywords

  • Chlorophyll-a
  • Energy-Transfer
  • Lutein
  • Micelles

ASJC Scopus subject areas

  • Medicine(all)

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