LSPR/TIRE bio-sensing platform for detection of low molecular weight toxins

Ali Al-Rubaye, Alexei Nabok, Hisham Abu-Ali, A. Székács, Ester Takacs

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

This work is a part of ongoing NATO SPS research project dedicated to optical detection of bio-toxins, particularly mycotoxins. One of the detection technologies chosen is a combination of localized surface plasmon resonance (LSPR) transducer with direct immunoassay with specific bio-receptors immobilized on the surface. The LSPR platform is based on gold nano-islands produced by thermal annealing of thin gold films deposited on glass. The gold nano-structures produced were analysed with SEM, AFM, optical absorption spectroscopy, and spectroscopic ellipsometry. The method of total internal reflection ellipsometry (TIRE) was chosen for LSPR bio-sensing because of its superior sensitivity as compared to conventional UV-vis absorption spectroscopy. The sensitivity of LSPR measurements is however limited by a finite evanescent decay length in gold nano-islands which is typically in the range of tens of nanometres. In order to achieve the best results, the bioreceptors must be small and placed as close to the gold surface as possible. In our case the problem was solved using either halved antibodies or aptamers immobilized covalently on the surface of gold. A series of bio-sensing experiments on detection of mycotoxins, i. e. aflatoxin B1, zearalenone, and ochratoxin A, went successfully and resulted in detection of the above mycotoxins in concentrations down to 10 ppt.

Original languageEnglish
Title of host publicationIEEE SENSORS 2017 - Conference Proceedings
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages1-3
Number of pages3
Volume2017-December
ISBN (Electronic)9781509010127
DOIs
Publication statusPublished - Dec 21 2017
Event16th IEEE SENSORS Conference, ICSENS 2017 - Glasgow, United Kingdom
Duration: Oct 30 2017Nov 1 2017

Other

Other16th IEEE SENSORS Conference, ICSENS 2017
CountryUnited Kingdom
CityGlasgow
Period10/30/1711/1/17

Fingerprint

Ellipsometry
Surface plasmon resonance
Gold
Molecular weight
Absorption spectroscopy
Aflatoxins
Spectroscopic ellipsometry
Ultraviolet spectroscopy
Antibodies
Light absorption
Transducers
Annealing
Glass
Scanning electron microscopy
Experiments

Keywords

  • gold nano-structures
  • LSPR
  • mycotoxins
  • TIRE

ASJC Scopus subject areas

  • Electrical and Electronic Engineering

Cite this

Al-Rubaye, A., Nabok, A., Abu-Ali, H., Székács, A., & Takacs, E. (2017). LSPR/TIRE bio-sensing platform for detection of low molecular weight toxins. In IEEE SENSORS 2017 - Conference Proceedings (Vol. 2017-December, pp. 1-3). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/ICSENS.2017.8234116

LSPR/TIRE bio-sensing platform for detection of low molecular weight toxins. / Al-Rubaye, Ali; Nabok, Alexei; Abu-Ali, Hisham; Székács, A.; Takacs, Ester.

IEEE SENSORS 2017 - Conference Proceedings. Vol. 2017-December Institute of Electrical and Electronics Engineers Inc., 2017. p. 1-3.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Al-Rubaye, A, Nabok, A, Abu-Ali, H, Székács, A & Takacs, E 2017, LSPR/TIRE bio-sensing platform for detection of low molecular weight toxins. in IEEE SENSORS 2017 - Conference Proceedings. vol. 2017-December, Institute of Electrical and Electronics Engineers Inc., pp. 1-3, 16th IEEE SENSORS Conference, ICSENS 2017, Glasgow, United Kingdom, 10/30/17. https://doi.org/10.1109/ICSENS.2017.8234116
Al-Rubaye A, Nabok A, Abu-Ali H, Székács A, Takacs E. LSPR/TIRE bio-sensing platform for detection of low molecular weight toxins. In IEEE SENSORS 2017 - Conference Proceedings. Vol. 2017-December. Institute of Electrical and Electronics Engineers Inc. 2017. p. 1-3 https://doi.org/10.1109/ICSENS.2017.8234116
Al-Rubaye, Ali ; Nabok, Alexei ; Abu-Ali, Hisham ; Székács, A. ; Takacs, Ester. / LSPR/TIRE bio-sensing platform for detection of low molecular weight toxins. IEEE SENSORS 2017 - Conference Proceedings. Vol. 2017-December Institute of Electrical and Electronics Engineers Inc., 2017. pp. 1-3
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