Low variability of internal transcribed spacer rDNA and trnL (UAA) intron sequences of several taxa in the Festuca ovina aggregate (Poaceae)

Z. Galli, K. Penksza, E. Kiss, L. Sági, L. Heszky

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Identification and classification of numerous Festuca species is still a difficult problem due to the close morphological resemblance. The most difficult fine fescues to identify belong to the Festuca ovina aggregate, which is the largest group in the genus Festuca. Many taxons are considered to be separate species based on quantitative taxonomic characters, differences in ploidy level or the structure of sclerenchyma cells. In order to evaluate the taxonomic value of DNA-based markers, sequence analysis of the internal transcribed spacer (ITS1-5.8S-ITS2) region and the chloroplast trnL (UAA) intron was performed in the ten most problematic fine fescues belonging to the Festuca ovina aggregate. Intraspecific ITS variants were found in a single case while in other cases only intragenomic ITS polymorphisms were detected with 1-2 ambiguous positions. Among the sequences of the trnL (UAA) intron even intragenomic polymorphisms were not detected in any of the Festuca species studied. Thus, the results do not support the species status of these ten taxa.

Original languageEnglish
Pages (from-to)57-69
Number of pages13
JournalActa Biologica Hungarica
Volume57
Issue number1
DOIs
Publication statusPublished - 2006

Fingerprint

Festuca
Festuca ovina
cell aggregates
Ribosomal DNA
Poaceae
Polymorphism
Introns
internal transcribed spacers
introns
polymorphism
ploidy
genetic polymorphism
DNA
sclerenchyma
chloroplast
Ploidies
Chloroplasts
Genetic Markers
Sequence Analysis
sequence analysis

Keywords

  • Festuca ovina aggregate
  • Internal transcribed spacer (ITS)
  • Molecular taxonomy
  • Ploidy level
  • trnL (UAA) intron

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Low variability of internal transcribed spacer rDNA and trnL (UAA) intron sequences of several taxa in the Festuca ovina aggregate (Poaceae). / Galli, Z.; Penksza, K.; Kiss, E.; Sági, L.; Heszky, L.

In: Acta Biologica Hungarica, Vol. 57, No. 1, 2006, p. 57-69.

Research output: Contribution to journalArticle

@article{b8b1ababb898473cb05859c11b215de9,
title = "Low variability of internal transcribed spacer rDNA and trnL (UAA) intron sequences of several taxa in the Festuca ovina aggregate (Poaceae)",
abstract = "Identification and classification of numerous Festuca species is still a difficult problem due to the close morphological resemblance. The most difficult fine fescues to identify belong to the Festuca ovina aggregate, which is the largest group in the genus Festuca. Many taxons are considered to be separate species based on quantitative taxonomic characters, differences in ploidy level or the structure of sclerenchyma cells. In order to evaluate the taxonomic value of DNA-based markers, sequence analysis of the internal transcribed spacer (ITS1-5.8S-ITS2) region and the chloroplast trnL (UAA) intron was performed in the ten most problematic fine fescues belonging to the Festuca ovina aggregate. Intraspecific ITS variants were found in a single case while in other cases only intragenomic ITS polymorphisms were detected with 1-2 ambiguous positions. Among the sequences of the trnL (UAA) intron even intragenomic polymorphisms were not detected in any of the Festuca species studied. Thus, the results do not support the species status of these ten taxa.",
keywords = "Festuca ovina aggregate, Internal transcribed spacer (ITS), Molecular taxonomy, Ploidy level, trnL (UAA) intron",
author = "Z. Galli and K. Penksza and E. Kiss and L. S{\'a}gi and L. Heszky",
year = "2006",
doi = "10.1556/ABiol.57.2006.1.6",
language = "English",
volume = "57",
pages = "57--69",
journal = "Acta Biologica Hungarica",
issn = "0236-5383",
publisher = "Akademiai Kiado",
number = "1",

}

TY - JOUR

T1 - Low variability of internal transcribed spacer rDNA and trnL (UAA) intron sequences of several taxa in the Festuca ovina aggregate (Poaceae)

AU - Galli, Z.

AU - Penksza, K.

AU - Kiss, E.

AU - Sági, L.

AU - Heszky, L.

PY - 2006

Y1 - 2006

N2 - Identification and classification of numerous Festuca species is still a difficult problem due to the close morphological resemblance. The most difficult fine fescues to identify belong to the Festuca ovina aggregate, which is the largest group in the genus Festuca. Many taxons are considered to be separate species based on quantitative taxonomic characters, differences in ploidy level or the structure of sclerenchyma cells. In order to evaluate the taxonomic value of DNA-based markers, sequence analysis of the internal transcribed spacer (ITS1-5.8S-ITS2) region and the chloroplast trnL (UAA) intron was performed in the ten most problematic fine fescues belonging to the Festuca ovina aggregate. Intraspecific ITS variants were found in a single case while in other cases only intragenomic ITS polymorphisms were detected with 1-2 ambiguous positions. Among the sequences of the trnL (UAA) intron even intragenomic polymorphisms were not detected in any of the Festuca species studied. Thus, the results do not support the species status of these ten taxa.

AB - Identification and classification of numerous Festuca species is still a difficult problem due to the close morphological resemblance. The most difficult fine fescues to identify belong to the Festuca ovina aggregate, which is the largest group in the genus Festuca. Many taxons are considered to be separate species based on quantitative taxonomic characters, differences in ploidy level or the structure of sclerenchyma cells. In order to evaluate the taxonomic value of DNA-based markers, sequence analysis of the internal transcribed spacer (ITS1-5.8S-ITS2) region and the chloroplast trnL (UAA) intron was performed in the ten most problematic fine fescues belonging to the Festuca ovina aggregate. Intraspecific ITS variants were found in a single case while in other cases only intragenomic ITS polymorphisms were detected with 1-2 ambiguous positions. Among the sequences of the trnL (UAA) intron even intragenomic polymorphisms were not detected in any of the Festuca species studied. Thus, the results do not support the species status of these ten taxa.

KW - Festuca ovina aggregate

KW - Internal transcribed spacer (ITS)

KW - Molecular taxonomy

KW - Ploidy level

KW - trnL (UAA) intron

UR - http://www.scopus.com/inward/record.url?scp=33745973280&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33745973280&partnerID=8YFLogxK

U2 - 10.1556/ABiol.57.2006.1.6

DO - 10.1556/ABiol.57.2006.1.6

M3 - Article

VL - 57

SP - 57

EP - 69

JO - Acta Biologica Hungarica

JF - Acta Biologica Hungarica

SN - 0236-5383

IS - 1

ER -