Low-pH induced reversible reorganizations of chloroplast thylakoid membranes — As revealed by small-angle neutron scattering

Renáta Ünnep, Ottó Zsiros, Zsolt Hörcsik, Márton Markó, Anjana Jajoo, Joachim Kohlbrecher, G. Garab, Gergely Nagy

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Energization of thylakoid membranes brings about the acidification of the lumenal aqueous phase, which activates important regulatory mechanisms. Earlier Jajoo and coworkers (2014 FEBS Lett. 588:970) have shown that low pH in isolated plant thylakoid membranes induces changes in the excitation energy distribution between the two photosystems. In order to elucidate the structural background of these changes, we used small-angle neutron scattering on thylakoid membranes exposed to low p2H (pD) and show that gradually lowering the p2H from 8.0 to 5.0 causes small but well discernible reversible diminishment of the periodic order and the lamellar repeat distance and an increased mosaicity — similar to the effects elicited by light-induced acidification of the lumen. Our data strongly suggest that thylakoids dynamically respond to the membrane energization and actively participate in different regulatory mechanisms.

Original languageEnglish
Pages (from-to)360-365
Number of pages6
JournalBiochimica et Biophysica Acta - Bioenergetics
Volume1858
Issue number5
DOIs
Publication statusPublished - May 1 2017

Fingerprint

Small Angle Scattering
Thylakoids
Neutrons
Chloroplasts
Neutron scattering
Membranes
Acidification
Excitation energy
Light

Keywords

  • Chloroplast thylakoid membranes
  • Lamellar repeat distance
  • Low pH and pH
  • Small-angle neutron scattering (SANS)

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

Cite this

Low-pH induced reversible reorganizations of chloroplast thylakoid membranes — As revealed by small-angle neutron scattering. / Ünnep, Renáta; Zsiros, Ottó; Hörcsik, Zsolt; Markó, Márton; Jajoo, Anjana; Kohlbrecher, Joachim; Garab, G.; Nagy, Gergely.

In: Biochimica et Biophysica Acta - Bioenergetics, Vol. 1858, No. 5, 01.05.2017, p. 360-365.

Research output: Contribution to journalArticle

Ünnep, Renáta ; Zsiros, Ottó ; Hörcsik, Zsolt ; Markó, Márton ; Jajoo, Anjana ; Kohlbrecher, Joachim ; Garab, G. ; Nagy, Gergely. / Low-pH induced reversible reorganizations of chloroplast thylakoid membranes — As revealed by small-angle neutron scattering. In: Biochimica et Biophysica Acta - Bioenergetics. 2017 ; Vol. 1858, No. 5. pp. 360-365.
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AU - Markó, Márton

AU - Jajoo, Anjana

AU - Kohlbrecher, Joachim

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