Low-frequency ultrasound for biofilm disruption in chronic rhinosinusitis with nasal polyposis

In vitro pilot study

T. Karosi, I. Sziklai, Péter Csomor

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Objectives/Hypothesis: Microbial biofilms have been implicated in the pathogenesis of chronic rhinosinusitis with nasal polyposis (CRSwNP). Although biofilms are characterized by an extremely high resistance against chemical and physical agents, low-frequency ultrasound (LFU) treatment has been suspected to be an efficient and safe method for biofilm disruption. Study Design: Basic science experimental study. Methods: A total of 10 patients with CRSwNP undergoing endoscopic sinus surgery were analyzed. Two series of identical nasal polyps (n = 20) were processed to hematoxylin-eosin (HE) and Gram staining and to continuous-wave LFU treatment (5 minutes, 0.4 MHz, 37°C), respectively. Results: Presence of microbial biofilms was confirmed in all patients with CRSwNP. HE staining showed a strong correlation with the results of Gram protocol in biofilm detection. In the LFU-treated group (n = 10), a significantly decreased inflammatory cell count was found in the subepithelial layer of nasal polyps (P

Original languageEnglish
Pages (from-to)17-23
Number of pages7
JournalLaryngoscope
Volume123
Issue number1
DOIs
Publication statusPublished - Jan 2013

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Biofilms
Nose
Nasal Polyps
Hematoxylin
Eosine Yellowish-(YS)
Staining and Labeling
Cell Count
In Vitro Techniques
Therapeutics

Keywords

  • Biofilm
  • chronic rhinosinusitis
  • low-frequency ultrasound
  • nasal polyps

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Low-frequency ultrasound for biofilm disruption in chronic rhinosinusitis with nasal polyposis : In vitro pilot study. / Karosi, T.; Sziklai, I.; Csomor, Péter.

In: Laryngoscope, Vol. 123, No. 1, 01.2013, p. 17-23.

Research output: Contribution to journalArticle

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