Low-Dose Irradiation and Short-Exposure Suboptimal-Dose Paclitaxel Adversely Modulate Metastatic Potential of Squamous Carcinoma Cells

József Lövey, Károly Fazekas, Andrea Ladányi, György Németh, József Tímár

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Background and Purpose: Low-dose irradiation and suboptimal drug concentrations may induce unexpected biological responses. Paclitaxel (PTX) is a widely used drug, which has a range of antitumoral effects and which is also regarded as a radiation sensitizer. In this study, it was tested how "suboptimal" short exposure to PTX modifies the biological effects of low-dose irradiation on human epithelial carcinoma cell lines. Material and Methods: 2 Gy irradiation, 7 and 100 nM PTX treatment, and their combinations were tested on squamous and transitional cell carcinoma lines A431, KB and ECV304. Cytoskeleton of interphase cells was investigated with immunocytochemistry and confocal laser scanning microscopy; viability and clonogenicity were assessed with MTT test and standard clonogenic assay. Effects on tumor growth and metastatic potential of A431 cells were tested in vivo using a liver metastasis model in SCID mice. Results: Exposure of human tumor cells to irradiation induced bundling of microtubules, similar to PTX. Combined treatments suspended each other's effect independently of treatment combinations. Single or combination treatments with low-dose PTX did affect cell proliferation to no relevant extent in vitro or in vivo (primary tumor xenografts of A431 cells). However, exposure to irradiation of A431 ceLLs inhibited while 100 nM PTX stimulated their liver-metastatic potentiaL Combination of 100 nM PTX with irradiation suspended the effect of 100 nM PTX and exhibited the highest antimetastatic activity. Conclusion: Short exposure to PTX and irradiation can interfere with each other's effects on tumor cells without significant modulation of proliferation and tumorigenicity. However, PTX and irradiation may significantly modulate the metastatic property of tumor cells.

Original languageEnglish
Pages (from-to)812-818
Number of pages7
JournalStrahlentherapie und Onkologie
Volume179
Issue number12
DOIs
Publication statusPublished - Dec 2003

Fingerprint

Paclitaxel
Squamous Cell Carcinoma
Neoplasms
Radiation-Sensitizing Agents
Cell Line
SCID Mice
Transitional Cell Carcinoma
Liver
Interphase
Therapeutics
Cytoskeleton
Heterografts
Confocal Microscopy
Microtubules
Pharmaceutical Preparations
Epithelial Cells
Immunohistochemistry
Cell Proliferation
Neoplasm Metastasis
Carcinoma

Keywords

  • Low-dose irradiation
  • Metastasis
  • Paclitaxel
  • Proliferation

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Cancer Research
  • Radiological and Ultrasound Technology

Cite this

Low-Dose Irradiation and Short-Exposure Suboptimal-Dose Paclitaxel Adversely Modulate Metastatic Potential of Squamous Carcinoma Cells. / Lövey, József; Fazekas, Károly; Ladányi, Andrea; Németh, György; Tímár, József.

In: Strahlentherapie und Onkologie, Vol. 179, No. 12, 12.2003, p. 812-818.

Research output: Contribution to journalArticle

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AU - Németh, György

AU - Tímár, József

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