Low-dose dithranol treatment and tape stripping induce tolerance to dithranol in a mouse ear oedema model

L. Kemény, Á Farkas, A. Dobozy

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background: It is well known from clinical practice that repeated treatment with dithranol leads to the development of tolerance. Objectives: To investigate the characteristics and mechanism of such dithranol tolerance. Methods: The mouse ear was pretreated with a low dose of dithranol or croton oil or, in previously sensitized animals, with dinitrofluorobenzene (DNFB). Twenty-four hours later irritant dermatitis was elicited by painting the mouse ear with a high dose of dithranol, croton oil or DNFB, and the dermatitis was characterized by measurement of ear thickness. Results: Low-dose dithranol significantly suppressed dithranol-induced oedema, whereas it had no effect on croton oil- or DNFB-induced dermatitis, suggesting that dithranol-induced tolerance is specific. Tolerance to dithranol could not be induced by pretreatment of the mouse ear with a low dose of croton oil or DNFB. Mild tape stripping of the mouse ear also inhibited the inflammatory effect of dithranol applied 24 h later. Superoxide dismutase treatment abolished the tolerance-inducing effect of low-dose dithranol or stripping. Conclusions: These results suggest that superoxide anion radicals are involved not only in the inflammatory effect of dithranol, but also in the induction of tolerance.

Original languageEnglish
Pages (from-to)764-769
Number of pages6
JournalBritish Journal of Dermatology
Volume146
Issue number5
DOIs
Publication statusPublished - Jun 1 2002

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Keywords

  • Dithranol
  • Superoxide dismutase
  • Tape stripping
  • Tolerance

ASJC Scopus subject areas

  • Dermatology

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