Low CD23 expression correlates with high CD38 expression and the presence of trisomy 12 in CLL

Csilla Kriston, Csaba Bödör, Kálmán Szenthe, Ferenc Bánáti, Barbara Bánkuti, B. Csernus, Lilla Reiniger, Judit Csomor, A. Matolcsy, G. Barna

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Chronic lymphocytic leukemia (CLL) is characterized by a neoplastic B-cell population coexpressing CD5 and CD23; however, the expression of CD23 is variable. In human, two isotypes of CD23 have been identified and related to different functions. The aim of our study was to investigate the relative expression of the two CD23 isotypes in CLL and find possible correlation with other prognostic factors. The expression of CD23 isotypes was analyzed in 54 cases of CLL by polymerase chain reaction (PCR) and quantitative real-time PCR. The immunophenotype of CLL cells was characterized by flow cytometry. We demonstrated a higher CD23a than CD23b expression of CLL cells. Our results also revealed two subsets of CLL cases with a distinct CD23 isotype expression pattern. Thirty-two percent of the cases (group CLL1) showed both low mRNA level of CD23 isotypes and high protein levels of CD20 and CD38 in contrast to group CLL2 with high CD23 mRNA levels. By correlating these results to the presence of prognostic factors determined by fluorescence in situ hybridization, we found that the majority of the cases of group CLL1 (14/17) carried trisomy 12. In summary, our results confirm a high CD23a/CD23b ratio of the CLL cells and demonstrate that in a subset of CLL cases, low CD23 expression together with high CD20 and CD38 expressions may serve as a surrogate for trisomy 12.

Original languageEnglish
JournalHematological Oncology
DOIs
Publication statusAccepted/In press - 2015

Fingerprint

Trisomy
B-Cell Chronic Lymphocytic Leukemia
Messenger RNA
Fluorescence In Situ Hybridization
Real-Time Polymerase Chain Reaction
Flow Cytometry
B-Lymphocytes
Polymerase Chain Reaction
Population

Keywords

  • CD23
  • CD38
  • CLL
  • Trisomy 12

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

Cite this

Low CD23 expression correlates with high CD38 expression and the presence of trisomy 12 in CLL. / Kriston, Csilla; Bödör, Csaba; Szenthe, Kálmán; Bánáti, Ferenc; Bánkuti, Barbara; Csernus, B.; Reiniger, Lilla; Csomor, Judit; Matolcsy, A.; Barna, G.

In: Hematological Oncology, 2015.

Research output: Contribution to journalArticle

Kriston, Csilla ; Bödör, Csaba ; Szenthe, Kálmán ; Bánáti, Ferenc ; Bánkuti, Barbara ; Csernus, B. ; Reiniger, Lilla ; Csomor, Judit ; Matolcsy, A. ; Barna, G. / Low CD23 expression correlates with high CD38 expression and the presence of trisomy 12 in CLL. In: Hematological Oncology. 2015.
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AU - Kriston, Csilla

AU - Bödör, Csaba

AU - Szenthe, Kálmán

AU - Bánáti, Ferenc

AU - Bánkuti, Barbara

AU - Csernus, B.

AU - Reiniger, Lilla

AU - Csomor, Judit

AU - Matolcsy, A.

AU - Barna, G.

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AB - Chronic lymphocytic leukemia (CLL) is characterized by a neoplastic B-cell population coexpressing CD5 and CD23; however, the expression of CD23 is variable. In human, two isotypes of CD23 have been identified and related to different functions. The aim of our study was to investigate the relative expression of the two CD23 isotypes in CLL and find possible correlation with other prognostic factors. The expression of CD23 isotypes was analyzed in 54 cases of CLL by polymerase chain reaction (PCR) and quantitative real-time PCR. The immunophenotype of CLL cells was characterized by flow cytometry. We demonstrated a higher CD23a than CD23b expression of CLL cells. Our results also revealed two subsets of CLL cases with a distinct CD23 isotype expression pattern. Thirty-two percent of the cases (group CLL1) showed both low mRNA level of CD23 isotypes and high protein levels of CD20 and CD38 in contrast to group CLL2 with high CD23 mRNA levels. By correlating these results to the presence of prognostic factors determined by fluorescence in situ hybridization, we found that the majority of the cases of group CLL1 (14/17) carried trisomy 12. In summary, our results confirm a high CD23a/CD23b ratio of the CLL cells and demonstrate that in a subset of CLL cases, low CD23 expression together with high CD20 and CD38 expressions may serve as a surrogate for trisomy 12.

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