Abstract
Autophagy delivers cytoplasmic material for lysosomal degradation in eukaryotic cells. Starvation induces high levels of autophagy to promote survival in the lack of nutrients. We compared genome-wide transcriptional profiles of fed and starved control, autophagy-deficient Atg7 and Atg1 null mutant Drosophila larvae to search for novel regulators of autophagy. Genes involved in catabolic processes including autophagy were transcriptionally upregulated in all cases. We also detected repression of genes involved in DNA replication in autophagy mutants compared with control animals. The expression of Rack1 (receptor of activated protein kinase C 1) increased 4.1- to 5.5-fold during nutrient deprivation in all three genotypes. The scaffold protein Rack1 plays a role in a wide range of processes including translation, cell adhesion and migration, cell survival and cancer. Loss of Rack1 led to attenuated autophagic response to starvation, and glycogen stores were decreased 11.8-fold in Rack1 mutant cells. Endogenous Rack1 partially colocalized with GFP-Atg8a and early autophagic structures on the ultrastructural level, suggesting its involvement in autophagosome formation. Endogenous Rack1 also showed a high degree of colocalization with glycogen particles in the larval fat body, and with Shaggy, the Drosophila homolog of glycogen synthase kinase 3B (GSK-3B). Our results, for the first time, demonstrated the fundamental role of Rack1 in autophagy and glycogen synthesis.
| Original language | English |
|---|---|
| Pages (from-to) | 1124-1135 |
| Number of pages | 12 |
| Journal | Autophagy |
| Volume | 8 |
| Issue number | 7 |
| DOIs | |
| Publication status | Published - Jul 2012 |
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Keywords
- Antimicrobial peptides
- Atg8
- Autophagy
- Drosophila
- Fat body
- Glycogen
- GSK-3B
- Microarray
- Rack1
- Starvation
ASJC Scopus subject areas
- Cell Biology
- Molecular Biology
Cite this
Loss of the starvation-induced gene Rack1 leads to glycogen deficiency and impaired autophagic responses in Drosophila. / Érdi, Balázs; Nagy, Péter; Zvara, A.; Varga, Ágnes; Pircs, Karolina; Ménesi, Dalma; Puskás, L.; Juhász, G.
In: Autophagy, Vol. 8, No. 7, 07.2012, p. 1124-1135.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Loss of the starvation-induced gene Rack1 leads to glycogen deficiency and impaired autophagic responses in Drosophila
AU - Érdi, Balázs
AU - Nagy, Péter
AU - Zvara, A.
AU - Varga, Ágnes
AU - Pircs, Karolina
AU - Ménesi, Dalma
AU - Puskás, L.
AU - Juhász, G.
PY - 2012/7
Y1 - 2012/7
N2 - Autophagy delivers cytoplasmic material for lysosomal degradation in eukaryotic cells. Starvation induces high levels of autophagy to promote survival in the lack of nutrients. We compared genome-wide transcriptional profiles of fed and starved control, autophagy-deficient Atg7 and Atg1 null mutant Drosophila larvae to search for novel regulators of autophagy. Genes involved in catabolic processes including autophagy were transcriptionally upregulated in all cases. We also detected repression of genes involved in DNA replication in autophagy mutants compared with control animals. The expression of Rack1 (receptor of activated protein kinase C 1) increased 4.1- to 5.5-fold during nutrient deprivation in all three genotypes. The scaffold protein Rack1 plays a role in a wide range of processes including translation, cell adhesion and migration, cell survival and cancer. Loss of Rack1 led to attenuated autophagic response to starvation, and glycogen stores were decreased 11.8-fold in Rack1 mutant cells. Endogenous Rack1 partially colocalized with GFP-Atg8a and early autophagic structures on the ultrastructural level, suggesting its involvement in autophagosome formation. Endogenous Rack1 also showed a high degree of colocalization with glycogen particles in the larval fat body, and with Shaggy, the Drosophila homolog of glycogen synthase kinase 3B (GSK-3B). Our results, for the first time, demonstrated the fundamental role of Rack1 in autophagy and glycogen synthesis.
AB - Autophagy delivers cytoplasmic material for lysosomal degradation in eukaryotic cells. Starvation induces high levels of autophagy to promote survival in the lack of nutrients. We compared genome-wide transcriptional profiles of fed and starved control, autophagy-deficient Atg7 and Atg1 null mutant Drosophila larvae to search for novel regulators of autophagy. Genes involved in catabolic processes including autophagy were transcriptionally upregulated in all cases. We also detected repression of genes involved in DNA replication in autophagy mutants compared with control animals. The expression of Rack1 (receptor of activated protein kinase C 1) increased 4.1- to 5.5-fold during nutrient deprivation in all three genotypes. The scaffold protein Rack1 plays a role in a wide range of processes including translation, cell adhesion and migration, cell survival and cancer. Loss of Rack1 led to attenuated autophagic response to starvation, and glycogen stores were decreased 11.8-fold in Rack1 mutant cells. Endogenous Rack1 partially colocalized with GFP-Atg8a and early autophagic structures on the ultrastructural level, suggesting its involvement in autophagosome formation. Endogenous Rack1 also showed a high degree of colocalization with glycogen particles in the larval fat body, and with Shaggy, the Drosophila homolog of glycogen synthase kinase 3B (GSK-3B). Our results, for the first time, demonstrated the fundamental role of Rack1 in autophagy and glycogen synthesis.
KW - Antimicrobial peptides
KW - Atg8
KW - Autophagy
KW - Drosophila
KW - Fat body
KW - Glycogen
KW - GSK-3B
KW - Microarray
KW - Rack1
KW - Starvation
UR - http://www.scopus.com/inward/record.url?scp=84866102456&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84866102456&partnerID=8YFLogxK
U2 - 10.4161/auto.20069
DO - 10.4161/auto.20069
M3 - Article
C2 - 22562043
AN - SCOPUS:84866102456
VL - 8
SP - 1124
EP - 1135
JO - Autophagy
JF - Autophagy
SN - 1554-8627
IS - 7
ER -