Loosening of the phage structure in a low ionic strength environment

G. Rontó, K. Tóth, G. Csík, L. A. Feigin, D. T. Svergun, A. T. Dembo, E. V. Shtikova

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Structural parameters of phage T7 were compared in two frequently use Tris buffers of high and low ionic strength, in order to explain the different biological activity and drug-binding characteristics. Characteristics of the whole phage geometry were obtained by viscosimetry, static and quasi-elastic light-scattering and small-angle X-ray scattering. The latter method revealed dissimilarities in the intraphage DNA compactness, consistent with the findings of the optical absorption melting studies. Alterations in the particle dimensions determined in the same sample by different methods are discussed, and a model is constructed to explain the structural modifications that occur on lowering the ionic strength.

Original languageEnglish
Pages (from-to)293-298
Number of pages6
JournalEuropean Biophysics Journal
Volume15
Issue number5
DOIs
Publication statusPublished - Feb 1988

Fingerprint

Bacteriophages
Osmolar Concentration
Bacteriophage T7
Tromethamine
Freezing
X-Rays
DNA
Pharmaceutical Preparations
Dynamic Light Scattering

Keywords

  • light-scattering
  • Nucleoprotein structure
  • small-angle X-ray scattering

ASJC Scopus subject areas

  • Biophysics

Cite this

Rontó, G., Tóth, K., Csík, G., Feigin, L. A., Svergun, D. T., Dembo, A. T., & Shtikova, E. V. (1988). Loosening of the phage structure in a low ionic strength environment. European Biophysics Journal, 15(5), 293-298. https://doi.org/10.1007/BF00256480

Loosening of the phage structure in a low ionic strength environment. / Rontó, G.; Tóth, K.; Csík, G.; Feigin, L. A.; Svergun, D. T.; Dembo, A. T.; Shtikova, E. V.

In: European Biophysics Journal, Vol. 15, No. 5, 02.1988, p. 293-298.

Research output: Contribution to journalArticle

Rontó, G, Tóth, K, Csík, G, Feigin, LA, Svergun, DT, Dembo, AT & Shtikova, EV 1988, 'Loosening of the phage structure in a low ionic strength environment', European Biophysics Journal, vol. 15, no. 5, pp. 293-298. https://doi.org/10.1007/BF00256480
Rontó, G. ; Tóth, K. ; Csík, G. ; Feigin, L. A. ; Svergun, D. T. ; Dembo, A. T. ; Shtikova, E. V. / Loosening of the phage structure in a low ionic strength environment. In: European Biophysics Journal. 1988 ; Vol. 15, No. 5. pp. 293-298.
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