Long-term effect of retinal ganglion cell axotomy on the histomorphometry of other cells in the porcine retina

András M. Komáromy, Dennis E. Brooks, Maria E. Källberg, William W. Dawson, A. Szél, Ákos Lukáts, Don A. Samuelson, Harold L. Sapp, Kirk N. Gelatt, Mark B. Sherwood

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Purpose: To determine the effect of retinal ganglion cell axotomy on the thickness of inner plexiform, inner nuclear, and outer plexiform layers, as well as the densities of short- and middle-to-long-wavelength cones, in the porcine retina. Methods: Unilateral retinal ganglion cell axotomy was performed in seven domestic pigs by either surgical optic nerve section or peripapillary argon laser photocoagulation. Damage to the retinal vasculature was ruled out with fluorescein angiography. Histologic examination of the retinal tissue was performed nine months later. Cone densities were determined immunohistochemically with the anti-visual pigment antibodies COS-1 and OS-2. Image analysis of semithin retinal cross sections was used to measure the thickness of the retinal layers. The effect of axotomy was quantified by optic nerve axon counts and estimations of retinal ganglion cell counts. The data were compared between the eyes with axotomy and the contralateral normal eye using the nonparametric Wilcoxon rank sum test. Results: Treatment of the peripapillary retina with the argon laser resulted in a median decrease in axon counts and retinal ganglion cell density estimates of 31%. No optic nerve axons and cells resembling retinal ganglion cells were found in the eyes with transected optic nerves. There was no significant difference in either the thickness of any retinal layers or cone densities between axotomized and normal control eyes. Conclusion: No signs of retrograde transsynaptic degeneration were observed in porcine retinas nine months after retinal ganglion cell axotomy.

Original languageEnglish
Pages (from-to)307-315
Number of pages9
JournalJournal of Glaucoma
Volume12
Issue number4
DOIs
Publication statusPublished - Aug 2003

Fingerprint

Axotomy
Retinal Ganglion Cells
Retina
Swine
Optic Nerve
Axons
Argon
Nonparametric Statistics
Lasers
Cell Count
Retrograde Degeneration
Sus scrofa
Retinal Pigments
Fluorescein Angiography
Light Coagulation
Neurons
Antibodies

Keywords

  • Axotomy
  • Pig
  • Retina
  • Transsynaptic degeneration

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Komáromy, A. M., Brooks, D. E., Källberg, M. E., Dawson, W. W., Szél, A., Lukáts, Á., ... Sherwood, M. B. (2003). Long-term effect of retinal ganglion cell axotomy on the histomorphometry of other cells in the porcine retina. Journal of Glaucoma, 12(4), 307-315. https://doi.org/10.1097/00061198-200308000-00004

Long-term effect of retinal ganglion cell axotomy on the histomorphometry of other cells in the porcine retina. / Komáromy, András M.; Brooks, Dennis E.; Källberg, Maria E.; Dawson, William W.; Szél, A.; Lukáts, Ákos; Samuelson, Don A.; Sapp, Harold L.; Gelatt, Kirk N.; Sherwood, Mark B.

In: Journal of Glaucoma, Vol. 12, No. 4, 08.2003, p. 307-315.

Research output: Contribution to journalArticle

Komáromy, AM, Brooks, DE, Källberg, ME, Dawson, WW, Szél, A, Lukáts, Á, Samuelson, DA, Sapp, HL, Gelatt, KN & Sherwood, MB 2003, 'Long-term effect of retinal ganglion cell axotomy on the histomorphometry of other cells in the porcine retina', Journal of Glaucoma, vol. 12, no. 4, pp. 307-315. https://doi.org/10.1097/00061198-200308000-00004
Komáromy, András M. ; Brooks, Dennis E. ; Källberg, Maria E. ; Dawson, William W. ; Szél, A. ; Lukáts, Ákos ; Samuelson, Don A. ; Sapp, Harold L. ; Gelatt, Kirk N. ; Sherwood, Mark B. / Long-term effect of retinal ganglion cell axotomy on the histomorphometry of other cells in the porcine retina. In: Journal of Glaucoma. 2003 ; Vol. 12, No. 4. pp. 307-315.
@article{77174745a12242cca4e4cbc2c60abe84,
title = "Long-term effect of retinal ganglion cell axotomy on the histomorphometry of other cells in the porcine retina",
abstract = "Purpose: To determine the effect of retinal ganglion cell axotomy on the thickness of inner plexiform, inner nuclear, and outer plexiform layers, as well as the densities of short- and middle-to-long-wavelength cones, in the porcine retina. Methods: Unilateral retinal ganglion cell axotomy was performed in seven domestic pigs by either surgical optic nerve section or peripapillary argon laser photocoagulation. Damage to the retinal vasculature was ruled out with fluorescein angiography. Histologic examination of the retinal tissue was performed nine months later. Cone densities were determined immunohistochemically with the anti-visual pigment antibodies COS-1 and OS-2. Image analysis of semithin retinal cross sections was used to measure the thickness of the retinal layers. The effect of axotomy was quantified by optic nerve axon counts and estimations of retinal ganglion cell counts. The data were compared between the eyes with axotomy and the contralateral normal eye using the nonparametric Wilcoxon rank sum test. Results: Treatment of the peripapillary retina with the argon laser resulted in a median decrease in axon counts and retinal ganglion cell density estimates of 31{\%}. No optic nerve axons and cells resembling retinal ganglion cells were found in the eyes with transected optic nerves. There was no significant difference in either the thickness of any retinal layers or cone densities between axotomized and normal control eyes. Conclusion: No signs of retrograde transsynaptic degeneration were observed in porcine retinas nine months after retinal ganglion cell axotomy.",
keywords = "Axotomy, Pig, Retina, Transsynaptic degeneration",
author = "Kom{\'a}romy, {Andr{\'a}s M.} and Brooks, {Dennis E.} and K{\"a}llberg, {Maria E.} and Dawson, {William W.} and A. Sz{\'e}l and {\'A}kos Luk{\'a}ts and Samuelson, {Don A.} and Sapp, {Harold L.} and Gelatt, {Kirk N.} and Sherwood, {Mark B.}",
year = "2003",
month = "8",
doi = "10.1097/00061198-200308000-00004",
language = "English",
volume = "12",
pages = "307--315",
journal = "Journal of Glaucoma",
issn = "1057-0829",
publisher = "Lippincott Williams and Wilkins",
number = "4",

}

TY - JOUR

T1 - Long-term effect of retinal ganglion cell axotomy on the histomorphometry of other cells in the porcine retina

AU - Komáromy, András M.

AU - Brooks, Dennis E.

AU - Källberg, Maria E.

AU - Dawson, William W.

AU - Szél, A.

AU - Lukáts, Ákos

AU - Samuelson, Don A.

AU - Sapp, Harold L.

AU - Gelatt, Kirk N.

AU - Sherwood, Mark B.

PY - 2003/8

Y1 - 2003/8

N2 - Purpose: To determine the effect of retinal ganglion cell axotomy on the thickness of inner plexiform, inner nuclear, and outer plexiform layers, as well as the densities of short- and middle-to-long-wavelength cones, in the porcine retina. Methods: Unilateral retinal ganglion cell axotomy was performed in seven domestic pigs by either surgical optic nerve section or peripapillary argon laser photocoagulation. Damage to the retinal vasculature was ruled out with fluorescein angiography. Histologic examination of the retinal tissue was performed nine months later. Cone densities were determined immunohistochemically with the anti-visual pigment antibodies COS-1 and OS-2. Image analysis of semithin retinal cross sections was used to measure the thickness of the retinal layers. The effect of axotomy was quantified by optic nerve axon counts and estimations of retinal ganglion cell counts. The data were compared between the eyes with axotomy and the contralateral normal eye using the nonparametric Wilcoxon rank sum test. Results: Treatment of the peripapillary retina with the argon laser resulted in a median decrease in axon counts and retinal ganglion cell density estimates of 31%. No optic nerve axons and cells resembling retinal ganglion cells were found in the eyes with transected optic nerves. There was no significant difference in either the thickness of any retinal layers or cone densities between axotomized and normal control eyes. Conclusion: No signs of retrograde transsynaptic degeneration were observed in porcine retinas nine months after retinal ganglion cell axotomy.

AB - Purpose: To determine the effect of retinal ganglion cell axotomy on the thickness of inner plexiform, inner nuclear, and outer plexiform layers, as well as the densities of short- and middle-to-long-wavelength cones, in the porcine retina. Methods: Unilateral retinal ganglion cell axotomy was performed in seven domestic pigs by either surgical optic nerve section or peripapillary argon laser photocoagulation. Damage to the retinal vasculature was ruled out with fluorescein angiography. Histologic examination of the retinal tissue was performed nine months later. Cone densities were determined immunohistochemically with the anti-visual pigment antibodies COS-1 and OS-2. Image analysis of semithin retinal cross sections was used to measure the thickness of the retinal layers. The effect of axotomy was quantified by optic nerve axon counts and estimations of retinal ganglion cell counts. The data were compared between the eyes with axotomy and the contralateral normal eye using the nonparametric Wilcoxon rank sum test. Results: Treatment of the peripapillary retina with the argon laser resulted in a median decrease in axon counts and retinal ganglion cell density estimates of 31%. No optic nerve axons and cells resembling retinal ganglion cells were found in the eyes with transected optic nerves. There was no significant difference in either the thickness of any retinal layers or cone densities between axotomized and normal control eyes. Conclusion: No signs of retrograde transsynaptic degeneration were observed in porcine retinas nine months after retinal ganglion cell axotomy.

KW - Axotomy

KW - Pig

KW - Retina

KW - Transsynaptic degeneration

UR - http://www.scopus.com/inward/record.url?scp=0141651943&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0141651943&partnerID=8YFLogxK

U2 - 10.1097/00061198-200308000-00004

DO - 10.1097/00061198-200308000-00004

M3 - Article

C2 - 12897575

AN - SCOPUS:0141651943

VL - 12

SP - 307

EP - 315

JO - Journal of Glaucoma

JF - Journal of Glaucoma

SN - 1057-0829

IS - 4

ER -