Long-term copper (Cu 2+) exposure impacts on auxin, nitric oxide (NO) metabolism and morphology of Arabidopsis thaliana L.

Zsuzsanna Kolbert, Andrea Peto, Nóra Lehotai, Gábor Feigl, L. Erdei

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Plants are able to dynamically adapt to their environment by reprogramming of their growth and development. Copper (Cu 2+) excess modifies shoot and root architecture of plants by a lesser known mechanism, therefore the involvement of a major hormone component (auxin) and a signal molecule (nitric oxide) in Cu 2+-induced morphological responses were studied in Arabidopsis using microscopic methods. Auxin-inducible gene expression was visualized in DR5::GUSArabidopsis and nitric oxide (NO) levels were detected by DAF-FM fluorophore in the stem and root system. Copper excess caused the inhibition of stem and root growth of Arabidopsis, during which cell elongation, division and expansion were also affected. The symptoms of stress-induced morphogenic response were found in the root system of 25 μM Cu 2+-treated plants. In both organs, the decrease of auxin-dependent gene expression was found, which can partly explain the growth inhibitions. Besides hormonal system, nitric oxide metabolism was also affected by Cu 2+. In root tips, copper excess induced NO generation, while NO content in lateral roots was not affected by the treatments. Using nia1nia2 mutants, nitrate reductase enzyme as a putative source of Cu 2+-induced NO was identified in Arabidopsis primary roots.

Original languageEnglish
Pages (from-to)151-159
Number of pages9
JournalPlant Growth Regulation
Volume68
Issue number2
DOIs
Publication statusPublished - Nov 2012

Fingerprint

Indoleacetic Acids
Arabidopsis
nitric oxide
Copper
auxins
Nitric Oxide
Arabidopsis thaliana
copper
metabolism
root systems
Gene Expression
Nitrate Reductase
gene expression
Plant Roots
Meristem
fluorescent dyes
plant architecture
stem elongation
Growth
nitrate reductase

Keywords

  • Auxin
  • Copper
  • Nitric oxide
  • Plant morphology

ASJC Scopus subject areas

  • Plant Science
  • Agronomy and Crop Science
  • Physiology

Cite this

Long-term copper (Cu 2+) exposure impacts on auxin, nitric oxide (NO) metabolism and morphology of Arabidopsis thaliana L. / Kolbert, Zsuzsanna; Peto, Andrea; Lehotai, Nóra; Feigl, Gábor; Erdei, L.

In: Plant Growth Regulation, Vol. 68, No. 2, 11.2012, p. 151-159.

Research output: Contribution to journalArticle

Kolbert, Zsuzsanna ; Peto, Andrea ; Lehotai, Nóra ; Feigl, Gábor ; Erdei, L. / Long-term copper (Cu 2+) exposure impacts on auxin, nitric oxide (NO) metabolism and morphology of Arabidopsis thaliana L. In: Plant Growth Regulation. 2012 ; Vol. 68, No. 2. pp. 151-159.
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