A stable association was established between the nitrogen-fixing bacterium Azotobacter vinelandii and the unicellular green alga Chlamydomonas reinhardtii on a carbon- and nitrogen-free medium. This medium ensured a balanced ratio between the two partners, as was detected by light and electron microscopy and a molecular biological method. The respiration, photosynthetic oxygen evolution and nitrogen fixation of the associative culture were lower compared with the data obtained from pure cultures growing under optimal conditions. However, observations confirmed a cooperation that improved over time, indicating a gradual stabilization of the system.
|Number of pages||6|
|Publication status||Published - Dec 1 2006|
- Alga-bacterium association
- Molecular detection
- Nitrogen fixation
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)