Location and quantification of metal ions in enzymes combining polyacrylamide gel electrophoresis and particle-induced X-ray emission

Zoltán Szökefalvi-Nagy, Csaba Bagyinka, István Demeter, Kornél L. Kovács, Huen Le Quynh

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

A method is presented to identify and determine the relative amounts of protein-bound metal ions in situ. Proteins or their subunits are directly scanned by a collimated proton beam of 3 MeV energy, and the characteristic X-rays produced are detected. The determination of Fe content of an iron-sulfur protein (HiPiP), as well as the Fe and Ni analysis of the hydrogenese from Thiocapsa roseopersina, have shown the feasibility of this technique.

Original languageEnglish
Pages (from-to)93-101
Number of pages9
JournalBiological Trace Element Research
Volume26-27
Issue number1
DOIs
Publication statusPublished - Jul 1 1990

Keywords

  • PIXE
  • bacterial hydrogenase
  • metalloenzyme
  • metalloprotein
  • particle-induced X-ray emission
  • polyacrylamide gel electrophoresis (PAGE)
  • trace element analysis

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical
  • Inorganic Chemistry

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