Localizations of inhibitors of the acetylcholine- and GABA-synthesizing systems in the rat brain - An autoradiographic assay on the distribution of 14C-hemicholinium and 14C-thiosemicarbazide

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Light microscopic autoradiographic techniques were employed to detect inhibitors of acetylcholine synthesis and of the GABA-metabolism in the rat nervous system. Sites of acetylcholine synthesis were located by demonstrating deposits of in vivo injected 14C-labelled hemicholinium; sites of GABA-metabolism were located by detecting in vivo injected 14C-labelled thiosemicarbazide. 14C-hemicholinium was concentrated in motor end plates, spinal motoneurons, cerebellar cortex, paleo- and neocortex cerebri, and caudate nucleus. Isotope thiosemicarbazide was found in the hippocampus, medial habenular nucleus, cerebellar cortex, dentate nucleus, substantia nigra and nuclei pontis. Localizations of the above enzyme inhibitors are in accord with biochemically demonstrated activities of choline acetylase and glutamic acid decarboxylase, respectively. It is concluded that the acetylcholine-synthesizing machinery is located partly in neuronal parikarya, but mainly in the neuropil. Sites of GABA-metabolism are more variable; they include neuronal parikarya, neuropil and also glial cells, with special predilection to various brain areas.

Original languageEnglish
Pages (from-to)1-16
Number of pages16
JournalExperimental Brain Research
Volume11
Issue number1
DOIs
Publication statusPublished - Aug 1970

Fingerprint

Hemicholinium 3
gamma-Aminobutyric Acid
Acetylcholine
Cerebellar Cortex
Neuropil
Brain
Motor Endplate
Habenula
Cerebellar Nuclei
Glutamate Decarboxylase
Choline O-Acetyltransferase
Caudate Nucleus
Neocortex
Motor Neurons
Enzyme Inhibitors
Substantia Nigra
Neuroglia
Isotopes
Cerebral Cortex
Nervous System

Keywords

  • Acetylcholine
  • Autoradiography
  • GABA
  • Hemicholinium
  • Thiosemicarbazide

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

@article{1261d7c88a5a426580afb082b7b6750c,
title = "Localizations of inhibitors of the acetylcholine- and GABA-synthesizing systems in the rat brain - An autoradiographic assay on the distribution of 14C-hemicholinium and 14C-thiosemicarbazide",
abstract = "Light microscopic autoradiographic techniques were employed to detect inhibitors of acetylcholine synthesis and of the GABA-metabolism in the rat nervous system. Sites of acetylcholine synthesis were located by demonstrating deposits of in vivo injected 14C-labelled hemicholinium; sites of GABA-metabolism were located by detecting in vivo injected 14C-labelled thiosemicarbazide. 14C-hemicholinium was concentrated in motor end plates, spinal motoneurons, cerebellar cortex, paleo- and neocortex cerebri, and caudate nucleus. Isotope thiosemicarbazide was found in the hippocampus, medial habenular nucleus, cerebellar cortex, dentate nucleus, substantia nigra and nuclei pontis. Localizations of the above enzyme inhibitors are in accord with biochemically demonstrated activities of choline acetylase and glutamic acid decarboxylase, respectively. It is concluded that the acetylcholine-synthesizing machinery is located partly in neuronal parikarya, but mainly in the neuropil. Sites of GABA-metabolism are more variable; they include neuronal parikarya, neuropil and also glial cells, with special predilection to various brain areas.",
keywords = "Acetylcholine, Autoradiography, GABA, Hemicholinium, Thiosemicarbazide",
author = "E. Knyih{\'a}r-Csillik and B. Csillik",
year = "1970",
month = "8",
doi = "10.1007/BF00234198",
language = "English",
volume = "11",
pages = "1--16",
journal = "Experimental Brain Research",
issn = "0014-4819",
publisher = "Springer Verlag",
number = "1",

}

TY - JOUR

T1 - Localizations of inhibitors of the acetylcholine- and GABA-synthesizing systems in the rat brain - An autoradiographic assay on the distribution of 14C-hemicholinium and 14C-thiosemicarbazide

AU - Knyihár-Csillik, E.

AU - Csillik, B.

PY - 1970/8

Y1 - 1970/8

N2 - Light microscopic autoradiographic techniques were employed to detect inhibitors of acetylcholine synthesis and of the GABA-metabolism in the rat nervous system. Sites of acetylcholine synthesis were located by demonstrating deposits of in vivo injected 14C-labelled hemicholinium; sites of GABA-metabolism were located by detecting in vivo injected 14C-labelled thiosemicarbazide. 14C-hemicholinium was concentrated in motor end plates, spinal motoneurons, cerebellar cortex, paleo- and neocortex cerebri, and caudate nucleus. Isotope thiosemicarbazide was found in the hippocampus, medial habenular nucleus, cerebellar cortex, dentate nucleus, substantia nigra and nuclei pontis. Localizations of the above enzyme inhibitors are in accord with biochemically demonstrated activities of choline acetylase and glutamic acid decarboxylase, respectively. It is concluded that the acetylcholine-synthesizing machinery is located partly in neuronal parikarya, but mainly in the neuropil. Sites of GABA-metabolism are more variable; they include neuronal parikarya, neuropil and also glial cells, with special predilection to various brain areas.

AB - Light microscopic autoradiographic techniques were employed to detect inhibitors of acetylcholine synthesis and of the GABA-metabolism in the rat nervous system. Sites of acetylcholine synthesis were located by demonstrating deposits of in vivo injected 14C-labelled hemicholinium; sites of GABA-metabolism were located by detecting in vivo injected 14C-labelled thiosemicarbazide. 14C-hemicholinium was concentrated in motor end plates, spinal motoneurons, cerebellar cortex, paleo- and neocortex cerebri, and caudate nucleus. Isotope thiosemicarbazide was found in the hippocampus, medial habenular nucleus, cerebellar cortex, dentate nucleus, substantia nigra and nuclei pontis. Localizations of the above enzyme inhibitors are in accord with biochemically demonstrated activities of choline acetylase and glutamic acid decarboxylase, respectively. It is concluded that the acetylcholine-synthesizing machinery is located partly in neuronal parikarya, but mainly in the neuropil. Sites of GABA-metabolism are more variable; they include neuronal parikarya, neuropil and also glial cells, with special predilection to various brain areas.

KW - Acetylcholine

KW - Autoradiography

KW - GABA

KW - Hemicholinium

KW - Thiosemicarbazide

UR - http://www.scopus.com/inward/record.url?scp=0014717872&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0014717872&partnerID=8YFLogxK

U2 - 10.1007/BF00234198

DO - 10.1007/BF00234198

M3 - Article

C2 - 5458715

AN - SCOPUS:0014717872

VL - 11

SP - 1

EP - 16

JO - Experimental Brain Research

JF - Experimental Brain Research

SN - 0014-4819

IS - 1

ER -