Localization of transglutaminase in human lenses

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Transglutaminase activity has been detected in the lenses of laboratory animals and in human cataracts. However, its distribution in the lens tissue has not been investigated. Using a monoclonal antibody against tissue transglutaminase, we showed by Western blotting and immunoabsorption that transglutaminase of normal human lens is immunologically related to tissue transglutaminase but has a slightly higher M(r) than the latter enzyme. Using monoclonal or polyclonal antibody against tissue transglutaminase, lens transglutaminase was localized to the epithelial cell layer on the anterior lens surface and to a thin stripe between the capsule and the peripheral cortex on the posterior surface. Lens fibers were not stained with the antibodies. Factor XIII, another transglutaminase, could not be detected in the lens tissue. The localization of transglutaminase in the lens suggests that lens transglutaminase is synthesized in the epithelial cells and secreted into the virtual space between the capsule and the peripheral cortex spreading all around the lens substance.

Original languageEnglish
Pages (from-to)1173-1177
Number of pages5
JournalJournal of Histochemistry and Cytochemistry
Volume43
Issue number11
Publication statusPublished - 1995

Fingerprint

Transglutaminases
Lenses
Capsules
Epithelial Cells
Factor XIII
Antibodies
Laboratory Animals
Cataract
Western Blotting
Monoclonal Antibodies

Keywords

  • Factor XIII
  • Human
  • Immunohistochemistry
  • Transglutaminase
  • Western blotting

ASJC Scopus subject areas

  • Cell Biology
  • Anatomy

Cite this

Localization of transglutaminase in human lenses. / Hidasi, V.; Ádány, R.; Muszbek, L.

In: Journal of Histochemistry and Cytochemistry, Vol. 43, No. 11, 1995, p. 1173-1177.

Research output: Contribution to journalArticle

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