Localization of glyceraldehyde-3-phosphate dehydrogenase in intact human erythrocytes. Evaluation of membrane adherence in autoradiographs at low grain density

M. Solti, F. Bartha, N. Halasz, G. Tóth, F. Sirokmán, P. Friedrich

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Abstract

Human erythrocytes were treated with highly tritiated [3H]iodoacetate under conditions when half of the label became attached to glyceraldehyde-3-phosphate dehydrogenase. After fixation, the cells were subjected to electron microscopic autoradiography. For the evaluation of distribution of grains, which were relatively few, a computerized method was developed. Statistical analysis of data showed the significant adherence of grains to the cell membrane. The results support the view that glyceraldehyde-3-phosphate dehydrogenase is localized near the membrane in the intact erythrocyte.

Original languageEnglish
Pages (from-to)9260-9265
Number of pages6
JournalJournal of Biological Chemistry
Volume256
Issue number17
Publication statusPublished - 1981

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Glyceraldehyde-3-Phosphate Dehydrogenases
Erythrocytes
Iodoacetates
Membranes
Statistical Data Interpretation
Cell membranes
Autoradiography
Labels
Statistical methods
Cell Membrane
Electrons

ASJC Scopus subject areas

  • Biochemistry

Cite this

Localization of glyceraldehyde-3-phosphate dehydrogenase in intact human erythrocytes. Evaluation of membrane adherence in autoradiographs at low grain density. / Solti, M.; Bartha, F.; Halasz, N.; Tóth, G.; Sirokmán, F.; Friedrich, P.

In: Journal of Biological Chemistry, Vol. 256, No. 17, 1981, p. 9260-9265.

Research output: Contribution to journalArticle

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AU - Sirokmán, F.

AU - Friedrich, P.

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