Localization of GABA-like immunoreactivity in the ectostriatum of domestic chicks: GABA immunocytochemistry combined with Golgi impregnation

A. Csillág, R. C. Bourne, Sanjay N. Patel, Michael G. Stewart, Teréz Tömböl

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The distribution of GABA-like immunoreactivity (GABA-LI) in the ectostriatal core (Ec) of domestic chicks (one to two days old) was investigated using (1) preembedding GABA immunocytochemistry and (2) Golgi impregnation and gold-toning combined with postembedding GABA immunocytochemistry. Two major classes of neurons which display GABA-LI were identified in chick Ec. Firstly, large GABA immunopositive cells which comprise at least two further subtypes: an ovoid or polygonal form of 14-18 μm diameter with no apparent polarity of dendrites and a smaller cell (10-14 (μm) with ovoid or basket-shaped soma and often more polarized dendritic ramification. In both subtypes the dendritic surface is smooth or sparsely spiny. Secondly, a small GABA immunopositive cell which is characterized by a round cell body of 5-8 μm diameter and thin and sparsely ramifying dendrites of smooth surface or with irregular protrusions. Based upon comprehensive descriptions of ectostriatal cytoarchitectonics (Tömböl etal., 1988c), and synaptology (Watanabe et al., 1985), we argue that the GABA-immunopositive cell types of chick Ec are likely to represent inhibitory interneurons comparable with GABAergic inhibitory cell types described in mammalian visual cortex.

Original languageEnglish
Pages (from-to)369-379
Number of pages11
JournalJournal of Neurocytology
Volume18
Issue number3
DOIs
Publication statusPublished - Jun 1989

Fingerprint

gamma-Aminobutyric Acid
Immunohistochemistry
Dendrites
Carisoprodol
Interneurons
Visual Cortex
Gold
Neurons

ASJC Scopus subject areas

  • Neuroscience(all)
  • Histology
  • Anatomy
  • Cell Biology

Cite this

Localization of GABA-like immunoreactivity in the ectostriatum of domestic chicks : GABA immunocytochemistry combined with Golgi impregnation. / Csillág, A.; Bourne, R. C.; Patel, Sanjay N.; Stewart, Michael G.; Tömböl, Teréz.

In: Journal of Neurocytology, Vol. 18, No. 3, 06.1989, p. 369-379.

Research output: Contribution to journalArticle

Csillág, A. ; Bourne, R. C. ; Patel, Sanjay N. ; Stewart, Michael G. ; Tömböl, Teréz. / Localization of GABA-like immunoreactivity in the ectostriatum of domestic chicks : GABA immunocytochemistry combined with Golgi impregnation. In: Journal of Neurocytology. 1989 ; Vol. 18, No. 3. pp. 369-379.
@article{e41e7cf879704dfe897fc35fa313a2b4,
title = "Localization of GABA-like immunoreactivity in the ectostriatum of domestic chicks: GABA immunocytochemistry combined with Golgi impregnation",
abstract = "The distribution of GABA-like immunoreactivity (GABA-LI) in the ectostriatal core (Ec) of domestic chicks (one to two days old) was investigated using (1) preembedding GABA immunocytochemistry and (2) Golgi impregnation and gold-toning combined with postembedding GABA immunocytochemistry. Two major classes of neurons which display GABA-LI were identified in chick Ec. Firstly, large GABA immunopositive cells which comprise at least two further subtypes: an ovoid or polygonal form of 14-18 μm diameter with no apparent polarity of dendrites and a smaller cell (10-14 (μm) with ovoid or basket-shaped soma and often more polarized dendritic ramification. In both subtypes the dendritic surface is smooth or sparsely spiny. Secondly, a small GABA immunopositive cell which is characterized by a round cell body of 5-8 μm diameter and thin and sparsely ramifying dendrites of smooth surface or with irregular protrusions. Based upon comprehensive descriptions of ectostriatal cytoarchitectonics (T{\"o}mb{\"o}l etal., 1988c), and synaptology (Watanabe et al., 1985), we argue that the GABA-immunopositive cell types of chick Ec are likely to represent inhibitory interneurons comparable with GABAergic inhibitory cell types described in mammalian visual cortex.",
author = "A. Csill{\'a}g and Bourne, {R. C.} and Patel, {Sanjay N.} and Stewart, {Michael G.} and Ter{\'e}z T{\"o}mb{\"o}l",
year = "1989",
month = "6",
doi = "10.1007/BF01190840",
language = "English",
volume = "18",
pages = "369--379",
journal = "Journal of Neurocytology",
issn = "0300-4864",
publisher = "Kluwer Academic Publishers",
number = "3",

}

TY - JOUR

T1 - Localization of GABA-like immunoreactivity in the ectostriatum of domestic chicks

T2 - GABA immunocytochemistry combined with Golgi impregnation

AU - Csillág, A.

AU - Bourne, R. C.

AU - Patel, Sanjay N.

AU - Stewart, Michael G.

AU - Tömböl, Teréz

PY - 1989/6

Y1 - 1989/6

N2 - The distribution of GABA-like immunoreactivity (GABA-LI) in the ectostriatal core (Ec) of domestic chicks (one to two days old) was investigated using (1) preembedding GABA immunocytochemistry and (2) Golgi impregnation and gold-toning combined with postembedding GABA immunocytochemistry. Two major classes of neurons which display GABA-LI were identified in chick Ec. Firstly, large GABA immunopositive cells which comprise at least two further subtypes: an ovoid or polygonal form of 14-18 μm diameter with no apparent polarity of dendrites and a smaller cell (10-14 (μm) with ovoid or basket-shaped soma and often more polarized dendritic ramification. In both subtypes the dendritic surface is smooth or sparsely spiny. Secondly, a small GABA immunopositive cell which is characterized by a round cell body of 5-8 μm diameter and thin and sparsely ramifying dendrites of smooth surface or with irregular protrusions. Based upon comprehensive descriptions of ectostriatal cytoarchitectonics (Tömböl etal., 1988c), and synaptology (Watanabe et al., 1985), we argue that the GABA-immunopositive cell types of chick Ec are likely to represent inhibitory interneurons comparable with GABAergic inhibitory cell types described in mammalian visual cortex.

AB - The distribution of GABA-like immunoreactivity (GABA-LI) in the ectostriatal core (Ec) of domestic chicks (one to two days old) was investigated using (1) preembedding GABA immunocytochemistry and (2) Golgi impregnation and gold-toning combined with postembedding GABA immunocytochemistry. Two major classes of neurons which display GABA-LI were identified in chick Ec. Firstly, large GABA immunopositive cells which comprise at least two further subtypes: an ovoid or polygonal form of 14-18 μm diameter with no apparent polarity of dendrites and a smaller cell (10-14 (μm) with ovoid or basket-shaped soma and often more polarized dendritic ramification. In both subtypes the dendritic surface is smooth or sparsely spiny. Secondly, a small GABA immunopositive cell which is characterized by a round cell body of 5-8 μm diameter and thin and sparsely ramifying dendrites of smooth surface or with irregular protrusions. Based upon comprehensive descriptions of ectostriatal cytoarchitectonics (Tömböl etal., 1988c), and synaptology (Watanabe et al., 1985), we argue that the GABA-immunopositive cell types of chick Ec are likely to represent inhibitory interneurons comparable with GABAergic inhibitory cell types described in mammalian visual cortex.

UR - http://www.scopus.com/inward/record.url?scp=0024334184&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024334184&partnerID=8YFLogxK

U2 - 10.1007/BF01190840

DO - 10.1007/BF01190840

M3 - Article

C2 - 2746308

AN - SCOPUS:0024334184

VL - 18

SP - 369

EP - 379

JO - Journal of Neurocytology

JF - Journal of Neurocytology

SN - 0300-4864

IS - 3

ER -