Localization of endomorphin-2-like immunoreactivity in the rat medulla and spinal cord

Sheryl Martin-Schild, James E. Zadina, Arnold A. Gerall, Sandor Vigh, Abba J. Kastin

Research output: Contribution to journalArticle

132 Citations (Scopus)

Abstract

Endomorphin-1 (Tyr-Pro-Trp-Phe-NH2) and endomorphin-2 (Tyr-Pro-Phe- Phe-NH2) are endogenous ligands that have greater affinity and selectivity for the μ-opiate receptor than any other known mammalian peptide. A polyclonal antiserum, screened for specificity to endomorphin-2 by immunodot- blot assay and preabsorption controls, was used for localization of this peptide. Immunocytochemistry performed on the brainstem, spinal cord, and sensory ganglia of rats by the avidin-biotin-peroxidase method revealed a continuous dense aggregation of endomorphin-2-like immunoreactive varicose fibers in the superficial laminae of the dorsal horn of the medulla and spinal cord. Immunoreactive fibers were detected in the dorsal root as well as within the dorsal root ganglia. The results suggest that endomorphin-2 is synthesized in primary sensory neurons in ganglia, transported to the superficial dorsal horn, and released near neurons expressing μ receptors. Its distribution appears to represent a functional unit likely to be associated with modulation of nociceptive stimuli.

Original languageEnglish
Pages (from-to)1641-1649
Number of pages9
JournalPeptides
Volume18
Issue number10
DOIs
Publication statusPublished - 1997

Fingerprint

Rats
Spinal Cord
Spinal Ganglia
Neurons
Sensory Ganglia
Peptides
Avidin
Fibers
Spinal Nerve Roots
Opioid Receptors
Sensory Receptor Cells
Biotin
Ganglia
Peroxidase
Brain Stem
Immune Sera
Assays
Agglomeration
Immunohistochemistry
Modulation

Keywords

  • Endomorphin
  • Immunocytochemistry
  • Opiate Spinal cord

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology
  • Cellular and Molecular Neuroscience

Cite this

Localization of endomorphin-2-like immunoreactivity in the rat medulla and spinal cord. / Martin-Schild, Sheryl; Zadina, James E.; Gerall, Arnold A.; Vigh, Sandor; Kastin, Abba J.

In: Peptides, Vol. 18, No. 10, 1997, p. 1641-1649.

Research output: Contribution to journalArticle

Martin-Schild, Sheryl ; Zadina, James E. ; Gerall, Arnold A. ; Vigh, Sandor ; Kastin, Abba J. / Localization of endomorphin-2-like immunoreactivity in the rat medulla and spinal cord. In: Peptides. 1997 ; Vol. 18, No. 10. pp. 1641-1649.
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