Localisation of 26S proteasomes with different subunit composition in insect muscles undergoing programmed cell death

P. Lőw, R. A. Hastings, S. P. Dawson, M. Sass, M. A. Billett, R. J. Mayer, S. E. Reynolds

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The 26S proteasome is a large multisubunit complex involved in degrading both cytoplasmic and nuclear proteins. We have investigated the subcellular distribution of four regulatory ATPase subunits (S6 (TBP7/MS73), S6′ (TBP1), S7 (MSS1), and S10b (SUG2)) together with components of 20S proteasomes in the intersegmental muscles (ISM) of Manduca sexta during developmentally programmed cell death (PCD). Immunogold electron microscopy shows that S6 is located in the heterochromatic part of nuclei of ISM fibres. S6′ is present in degraded material only outside intact fibres. S7 can be detected in nuclei, cytoplasm and also in degraded material. S10b, on the other hand, is initially found in nuclei and subsequently in degraded cytoplasmic locations during PCD. 20S proteasomes are present in all areas where ATPase subunits are detected, consistent with the presence of intact 26S proteasomes. These results are discussed in terms of heterogeneity of 26S proteasomes, 26S proteasome disassembly and the possible role of ATPases in non-proteasome complexes in the process of PCD.

Original languageEnglish
Pages (from-to)1210-1217
Number of pages8
JournalCell Death and Differentiation
Volume7
Issue number12
DOIs
Publication statusPublished - 2000

Fingerprint

S 6
Insects
Cell Death
ethyl-2-methylthio-4-methyl-5-pyrimidine carboxylate
Adenosine Triphosphatases
Muscles
Proteasome Endopeptidase Complex
Manduca
Nuclear Proteins
Electron Microscopy
Cytoplasm
ATP dependent 26S protease

Keywords

  • Immunogold method
  • Manduca sexta
  • Muscle degradation
  • Programmed cell death
  • Proteasome regulatory ATPase subunit
  • Tobacco hornworm

ASJC Scopus subject areas

  • Cell Biology

Cite this

Localisation of 26S proteasomes with different subunit composition in insect muscles undergoing programmed cell death. / Lőw, P.; Hastings, R. A.; Dawson, S. P.; Sass, M.; Billett, M. A.; Mayer, R. J.; Reynolds, S. E.

In: Cell Death and Differentiation, Vol. 7, No. 12, 2000, p. 1210-1217.

Research output: Contribution to journalArticle

Lőw, P. ; Hastings, R. A. ; Dawson, S. P. ; Sass, M. ; Billett, M. A. ; Mayer, R. J. ; Reynolds, S. E. / Localisation of 26S proteasomes with different subunit composition in insect muscles undergoing programmed cell death. In: Cell Death and Differentiation. 2000 ; Vol. 7, No. 12. pp. 1210-1217.
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