Local postsynaptic voltage-gated sodium channel activation in dendritic spines of olfactory bulb granule cells

Wolfgang G. Bywalez, Dinu Patirniche, Vanessa Rupprecht, Martin Stemmler, Andreas V M Herz, Dénes Pálfi, B. Rózsa, Veronica Egger

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Neuronal dendritic spines have been speculated to function as independent computational units, yet evidence for active electrical computation in spines is scarce. Here we show that strictly local voltage-gated sodium channel (Nav) activation can occur during excitatory postsynaptic potentials in the spines of olfactory bulb granule cells, which we mimic and detect via combined two-photon uncaging of glutamate andcalcium imaging in conjunction with whole-cell recordings. We find that local Nav activation boosts calcium entry into spines through high-voltage-activated calcium channels and accelerates postsynaptic somatic depolarization, without affecting NMDA receptor-mediated signaling. Hence, Nav-mediated boosting promotes rapid output from the reciprocal granule cell spine onto the lateral mitral cell dendrite and thus can speed up recurrent inhibition. This striking example of electrical compartmentalization both adds to the understanding of olfactory network processing and broadens the general view of spine function.

Original languageEnglish
Pages (from-to)590-601
Number of pages12
JournalNeuron
Volume85
Issue number3
DOIs
Publication statusPublished - Feb 4 2015

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Voltage-Gated Sodium Channels
Dendritic Spines
Olfactory Bulb
Spine
Excitatory Postsynaptic Potentials
Patch-Clamp Techniques
Calcium Channels
Dendrites
N-Methyl-D-Aspartate Receptors
Photons
Glutamic Acid
Calcium

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Bywalez, W. G., Patirniche, D., Rupprecht, V., Stemmler, M., Herz, A. V. M., Pálfi, D., ... Egger, V. (2015). Local postsynaptic voltage-gated sodium channel activation in dendritic spines of olfactory bulb granule cells. Neuron, 85(3), 590-601. https://doi.org/10.1016/j.neuron.2014.12.051

Local postsynaptic voltage-gated sodium channel activation in dendritic spines of olfactory bulb granule cells. / Bywalez, Wolfgang G.; Patirniche, Dinu; Rupprecht, Vanessa; Stemmler, Martin; Herz, Andreas V M; Pálfi, Dénes; Rózsa, B.; Egger, Veronica.

In: Neuron, Vol. 85, No. 3, 04.02.2015, p. 590-601.

Research output: Contribution to journalArticle

Bywalez, WG, Patirniche, D, Rupprecht, V, Stemmler, M, Herz, AVM, Pálfi, D, Rózsa, B & Egger, V 2015, 'Local postsynaptic voltage-gated sodium channel activation in dendritic spines of olfactory bulb granule cells', Neuron, vol. 85, no. 3, pp. 590-601. https://doi.org/10.1016/j.neuron.2014.12.051
Bywalez WG, Patirniche D, Rupprecht V, Stemmler M, Herz AVM, Pálfi D et al. Local postsynaptic voltage-gated sodium channel activation in dendritic spines of olfactory bulb granule cells. Neuron. 2015 Feb 4;85(3):590-601. https://doi.org/10.1016/j.neuron.2014.12.051
Bywalez, Wolfgang G. ; Patirniche, Dinu ; Rupprecht, Vanessa ; Stemmler, Martin ; Herz, Andreas V M ; Pálfi, Dénes ; Rózsa, B. ; Egger, Veronica. / Local postsynaptic voltage-gated sodium channel activation in dendritic spines of olfactory bulb granule cells. In: Neuron. 2015 ; Vol. 85, No. 3. pp. 590-601.
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