Local γ-aminobutyric acid and glutamate circuit control of hypophyseotrophic corticotropin-releasing factor neuron activity in the paraventricular nucleus of the hypothalamus

V. Bartanusz, D. Muller, R. C. Gaillard, P. Streit, L. Vutskits, J. Kiss

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Paraventricular corticotropin-releasing factor (CRF) neurons play a pivotal role in regulating neuroendocrine responses to stress. The mechanisms by which synaptic inputs control the activity of these neurons are not well understood. The present study was undertaken to determine the role of the intrinsic γ-aminobutyric acid (GABA)- and glutamatergic neural circuits of the hypothalamic paraventricular nucleus (PVN) in the control of CRF neural activity. We show that in organotypic cultures of the PVN, blockade of the intrinsic GABAergic neurotransmission by the GABAA receptor antagonist bicuculline resulted in a significant increase in CRF secretion. The bicuculline-induced CRF secretory activity was abolished by the coadministration of the selective α-amino-3-hydroxy-5-methyl-4- isoxazoleprionic acid (AMPA)/kainate receptor antagonist 6-cyano-7- nitroquinoxaline-2,3-dione (CNQX). Electrical stimulation of the CRF cell division elicited glutamatergic extracellular field potentials that were dramatically enhanced by bicuculline and were suppressed by CNQX. These results show that the functional activity of CRF neurons in organotypic cultures of the PVN is under a tonic inhibitory influence of an intrinsic GABAergic circuit. Suppression of GABAergic transmission appears to have a permissive role for inducing an increased secretory activity of CRF neurons that is driven by an excitatory glutamatergic network via AMPA/kainate receptors.

Original languageEnglish
Pages (from-to)777-782
Number of pages6
JournalEuropean Journal of Neuroscience
Volume19
Issue number3
DOIs
Publication statusPublished - Feb 2004

Fingerprint

Aminobutyrates
Paraventricular Hypothalamic Nucleus
Corticotropin-Releasing Hormone
Hypothalamus
Glutamic Acid
Neurons
Bicuculline
6-Cyano-7-nitroquinoxaline-2,3-dione
Kainic Acid Receptors
AMPA Receptors
GABA-A Receptor Antagonists
Synaptic Transmission
Cell Division
gamma-Aminobutyric Acid
Electric Stimulation
Acids

Keywords

  • γ-aminobutyric acid
  • Bicuculline
  • Corticotropin-releasing factor
  • Glutamate
  • Paraventricular nucleus

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Local γ-aminobutyric acid and glutamate circuit control of hypophyseotrophic corticotropin-releasing factor neuron activity in the paraventricular nucleus of the hypothalamus. / Bartanusz, V.; Muller, D.; Gaillard, R. C.; Streit, P.; Vutskits, L.; Kiss, J.

In: European Journal of Neuroscience, Vol. 19, No. 3, 02.2004, p. 777-782.

Research output: Contribution to journalArticle

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