Liver regeneration after different degrees of portal vein ligation

David Tibor Lauber, Dóra Krisztina Tihanyi, Zoltán Czigány, T. Kovács, András Budai, Dóra Drozgyik, András Fülöp, Attila Szijártó

Research output: Contribution to journalArticle

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Abstract

Background Selective portal vein ligation (PVL) is followed by ipsilateral atrophy and contralateral hypertrophy of the liver lobes. Although the atrophy-hypertrophy complex induced by PVL is a well-documented phenomenon, the effect of different degrees of extended portal vein occlusion on liver regeneration is not known. The aim of this study was to assess the effects of different degrees of portal occlusion on portal pressure and liver regeneration. Materials and methods Male Wistar rats (n = 96; 220-250 g) were randomized into three groups and underwent 70%, 80%, or 90% portal vein ligation, respectively. The portal pressure was measured immediately and 24, 48, 72, 120, and 168 h after PVL (n = 6/group/time point). The hepatic lobes and the spleen were weighed, and liver regeneration ratio was calculated. Changes in liver histology and the mitotic activity were assessed on hematoxylin-eosin stained slides. Results Higher degree of portal occlusion triggered a stronger regenerative response (regeneration ratio of PVL 70%168h = 2.23 ± 0.13, PVL 80%168h = 3.11 ± 0.37, PVL 90%168h = 4.68 ± 0.48) PVL led to an immediate increase in portal pressure, the value of which changed proportionally to the mass of liver tissue deprived of portal perfusion (PVL 70%acute = 17 ± 2 mm Hg, PVL 80%acute = 19 ± 1 mm Hg, PVL 90%acute = 26 ± 4 mm Hg). Findings in histology showed necro-apoptotic lesions in the atrophic liver lobes and increased mitotic cell count in the hypertrophic lobes. The mitotic cell count of PVL 90% peaked earlier and at a significantly higher value than of PVL 70% and PVL 80% (PVL 9024h%: 96.0 ± 3.5 PVL 70%48h: 64.0 ± 2.1, PVL 80%48h: 56.3 ± 4.0). The mitotic index after 24 h showed a strong correlation with the acute portal hypertension. Conclusions A higher degree of portal vein occlusion leads to a greater regenerative response, presumably triggered by the proportional increase in portal pressure, which supports the role of the so-called "blood-flow" theory of PVL-triggered liver regeneration.

Original languageEnglish
Pages (from-to)451-458
Number of pages8
JournalJournal of Surgical Research
Volume203
Issue number2
DOIs
Publication statusPublished - Jun 15 2016

Fingerprint

Liver Regeneration
Portal Vein
Ligation
Portal Pressure
Liver
Hypertrophy
Atrophy
Histology
Cell Count
Mitotic Index

Keywords

  • Liver regeneration
  • Portal pressure
  • Portal vein ligation
  • Regeneration ratio

ASJC Scopus subject areas

  • Surgery

Cite this

Lauber, D. T., Tihanyi, D. K., Czigány, Z., Kovács, T., Budai, A., Drozgyik, D., ... Szijártó, A. (2016). Liver regeneration after different degrees of portal vein ligation. Journal of Surgical Research, 203(2), 451-458. https://doi.org/10.1016/j.jss.2016.03.032

Liver regeneration after different degrees of portal vein ligation. / Lauber, David Tibor; Tihanyi, Dóra Krisztina; Czigány, Zoltán; Kovács, T.; Budai, András; Drozgyik, Dóra; Fülöp, András; Szijártó, Attila.

In: Journal of Surgical Research, Vol. 203, No. 2, 15.06.2016, p. 451-458.

Research output: Contribution to journalArticle

Lauber, DT, Tihanyi, DK, Czigány, Z, Kovács, T, Budai, A, Drozgyik, D, Fülöp, A & Szijártó, A 2016, 'Liver regeneration after different degrees of portal vein ligation', Journal of Surgical Research, vol. 203, no. 2, pp. 451-458. https://doi.org/10.1016/j.jss.2016.03.032
Lauber, David Tibor ; Tihanyi, Dóra Krisztina ; Czigány, Zoltán ; Kovács, T. ; Budai, András ; Drozgyik, Dóra ; Fülöp, András ; Szijártó, Attila. / Liver regeneration after different degrees of portal vein ligation. In: Journal of Surgical Research. 2016 ; Vol. 203, No. 2. pp. 451-458.
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abstract = "Background Selective portal vein ligation (PVL) is followed by ipsilateral atrophy and contralateral hypertrophy of the liver lobes. Although the atrophy-hypertrophy complex induced by PVL is a well-documented phenomenon, the effect of different degrees of extended portal vein occlusion on liver regeneration is not known. The aim of this study was to assess the effects of different degrees of portal occlusion on portal pressure and liver regeneration. Materials and methods Male Wistar rats (n = 96; 220-250 g) were randomized into three groups and underwent 70{\%}, 80{\%}, or 90{\%} portal vein ligation, respectively. The portal pressure was measured immediately and 24, 48, 72, 120, and 168 h after PVL (n = 6/group/time point). The hepatic lobes and the spleen were weighed, and liver regeneration ratio was calculated. Changes in liver histology and the mitotic activity were assessed on hematoxylin-eosin stained slides. Results Higher degree of portal occlusion triggered a stronger regenerative response (regeneration ratio of PVL 70{\%}168h = 2.23 ± 0.13, PVL 80{\%}168h = 3.11 ± 0.37, PVL 90{\%}168h = 4.68 ± 0.48) PVL led to an immediate increase in portal pressure, the value of which changed proportionally to the mass of liver tissue deprived of portal perfusion (PVL 70{\%}acute = 17 ± 2 mm Hg, PVL 80{\%}acute = 19 ± 1 mm Hg, PVL 90{\%}acute = 26 ± 4 mm Hg). Findings in histology showed necro-apoptotic lesions in the atrophic liver lobes and increased mitotic cell count in the hypertrophic lobes. The mitotic cell count of PVL 90{\%} peaked earlier and at a significantly higher value than of PVL 70{\%} and PVL 80{\%} (PVL 9024h{\%}: 96.0 ± 3.5 PVL 70{\%}48h: 64.0 ± 2.1, PVL 80{\%}48h: 56.3 ± 4.0). The mitotic index after 24 h showed a strong correlation with the acute portal hypertension. Conclusions A higher degree of portal vein occlusion leads to a greater regenerative response, presumably triggered by the proportional increase in portal pressure, which supports the role of the so-called {"}blood-flow{"} theory of PVL-triggered liver regeneration.",
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AU - Tihanyi, Dóra Krisztina

AU - Czigány, Zoltán

AU - Kovács, T.

AU - Budai, András

AU - Drozgyik, Dóra

AU - Fülöp, András

AU - Szijártó, Attila

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N2 - Background Selective portal vein ligation (PVL) is followed by ipsilateral atrophy and contralateral hypertrophy of the liver lobes. Although the atrophy-hypertrophy complex induced by PVL is a well-documented phenomenon, the effect of different degrees of extended portal vein occlusion on liver regeneration is not known. The aim of this study was to assess the effects of different degrees of portal occlusion on portal pressure and liver regeneration. Materials and methods Male Wistar rats (n = 96; 220-250 g) were randomized into three groups and underwent 70%, 80%, or 90% portal vein ligation, respectively. The portal pressure was measured immediately and 24, 48, 72, 120, and 168 h after PVL (n = 6/group/time point). The hepatic lobes and the spleen were weighed, and liver regeneration ratio was calculated. Changes in liver histology and the mitotic activity were assessed on hematoxylin-eosin stained slides. Results Higher degree of portal occlusion triggered a stronger regenerative response (regeneration ratio of PVL 70%168h = 2.23 ± 0.13, PVL 80%168h = 3.11 ± 0.37, PVL 90%168h = 4.68 ± 0.48) PVL led to an immediate increase in portal pressure, the value of which changed proportionally to the mass of liver tissue deprived of portal perfusion (PVL 70%acute = 17 ± 2 mm Hg, PVL 80%acute = 19 ± 1 mm Hg, PVL 90%acute = 26 ± 4 mm Hg). Findings in histology showed necro-apoptotic lesions in the atrophic liver lobes and increased mitotic cell count in the hypertrophic lobes. The mitotic cell count of PVL 90% peaked earlier and at a significantly higher value than of PVL 70% and PVL 80% (PVL 9024h%: 96.0 ± 3.5 PVL 70%48h: 64.0 ± 2.1, PVL 80%48h: 56.3 ± 4.0). The mitotic index after 24 h showed a strong correlation with the acute portal hypertension. Conclusions A higher degree of portal vein occlusion leads to a greater regenerative response, presumably triggered by the proportional increase in portal pressure, which supports the role of the so-called "blood-flow" theory of PVL-triggered liver regeneration.

AB - Background Selective portal vein ligation (PVL) is followed by ipsilateral atrophy and contralateral hypertrophy of the liver lobes. Although the atrophy-hypertrophy complex induced by PVL is a well-documented phenomenon, the effect of different degrees of extended portal vein occlusion on liver regeneration is not known. The aim of this study was to assess the effects of different degrees of portal occlusion on portal pressure and liver regeneration. Materials and methods Male Wistar rats (n = 96; 220-250 g) were randomized into three groups and underwent 70%, 80%, or 90% portal vein ligation, respectively. The portal pressure was measured immediately and 24, 48, 72, 120, and 168 h after PVL (n = 6/group/time point). The hepatic lobes and the spleen were weighed, and liver regeneration ratio was calculated. Changes in liver histology and the mitotic activity were assessed on hematoxylin-eosin stained slides. Results Higher degree of portal occlusion triggered a stronger regenerative response (regeneration ratio of PVL 70%168h = 2.23 ± 0.13, PVL 80%168h = 3.11 ± 0.37, PVL 90%168h = 4.68 ± 0.48) PVL led to an immediate increase in portal pressure, the value of which changed proportionally to the mass of liver tissue deprived of portal perfusion (PVL 70%acute = 17 ± 2 mm Hg, PVL 80%acute = 19 ± 1 mm Hg, PVL 90%acute = 26 ± 4 mm Hg). Findings in histology showed necro-apoptotic lesions in the atrophic liver lobes and increased mitotic cell count in the hypertrophic lobes. The mitotic cell count of PVL 90% peaked earlier and at a significantly higher value than of PVL 70% and PVL 80% (PVL 9024h%: 96.0 ± 3.5 PVL 70%48h: 64.0 ± 2.1, PVL 80%48h: 56.3 ± 4.0). The mitotic index after 24 h showed a strong correlation with the acute portal hypertension. Conclusions A higher degree of portal vein occlusion leads to a greater regenerative response, presumably triggered by the proportional increase in portal pressure, which supports the role of the so-called "blood-flow" theory of PVL-triggered liver regeneration.

KW - Liver regeneration

KW - Portal pressure

KW - Portal vein ligation

KW - Regeneration ratio

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