Live observation of fission yeast meiosis in recombination-deficient mutants

A study on achiasmate chromosome segregation

M. Molnár, J. Bähler, J. Kohli, Y. Hiraoka

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Regular segregation of homologous chromosomes during meiotic divisions is essential for the generation of viable progeny. In recombination-proficient organisms, chromosome disjunction at meiosis I generally occurs by chiasma formation between the homologs (chiasmate meiosis). We have studied meiotic stages in living rec8 and rec7 mutant cells of fission yeast, with special attention to prophase and the first meiotic division. Both rec8 and rec7 are early recombination mutants, and in rec7 mutants, chromosome segregation at meiosis I occurs without any recombination (achiasmate meiosis). Both mutants showed distinct irregularities in nuclear prophase movements. Additionally, rec7 showed an extended first division of variable length and with single chromosomes changing back and forth between the cell poles. Two other early recombination deficient mutants (rec14 and rec15) showed very similar phenotypes to rec7 during the first meiotic division, and the fidelity of achiasmate chromosome segregation slightly exceeded the expected random level. We discuss possible regulatory mechanisms of fission yeast to deal with achiasmate chromosome segregation.

Original languageEnglish
Pages (from-to)2843-2853
Number of pages11
JournalJournal of Cell Science
Volume114
Issue number15
Publication statusPublished - 2001

Fingerprint

Chromosome Segregation
Schizosaccharomyces
Meiosis
Genetic Recombination
Observation
Prophase
Chromosomes
Phenotype

Keywords

  • Achiasmate segregation
  • Chromosome segregation
  • Fission yeast
  • Meiosis

ASJC Scopus subject areas

  • Cell Biology

Cite this

Live observation of fission yeast meiosis in recombination-deficient mutants : A study on achiasmate chromosome segregation. / Molnár, M.; Bähler, J.; Kohli, J.; Hiraoka, Y.

In: Journal of Cell Science, Vol. 114, No. 15, 2001, p. 2843-2853.

Research output: Contribution to journalArticle

@article{7144111adbf942d3ba9a68d055ef66f8,
title = "Live observation of fission yeast meiosis in recombination-deficient mutants: A study on achiasmate chromosome segregation",
abstract = "Regular segregation of homologous chromosomes during meiotic divisions is essential for the generation of viable progeny. In recombination-proficient organisms, chromosome disjunction at meiosis I generally occurs by chiasma formation between the homologs (chiasmate meiosis). We have studied meiotic stages in living rec8 and rec7 mutant cells of fission yeast, with special attention to prophase and the first meiotic division. Both rec8 and rec7 are early recombination mutants, and in rec7 mutants, chromosome segregation at meiosis I occurs without any recombination (achiasmate meiosis). Both mutants showed distinct irregularities in nuclear prophase movements. Additionally, rec7 showed an extended first division of variable length and with single chromosomes changing back and forth between the cell poles. Two other early recombination deficient mutants (rec14 and rec15) showed very similar phenotypes to rec7 during the first meiotic division, and the fidelity of achiasmate chromosome segregation slightly exceeded the expected random level. We discuss possible regulatory mechanisms of fission yeast to deal with achiasmate chromosome segregation.",
keywords = "Achiasmate segregation, Chromosome segregation, Fission yeast, Meiosis",
author = "M. Moln{\'a}r and J. B{\"a}hler and J. Kohli and Y. Hiraoka",
year = "2001",
language = "English",
volume = "114",
pages = "2843--2853",
journal = "Journal of Cell Science",
issn = "0021-9533",
publisher = "Company of Biologists Ltd",
number = "15",

}

TY - JOUR

T1 - Live observation of fission yeast meiosis in recombination-deficient mutants

T2 - A study on achiasmate chromosome segregation

AU - Molnár, M.

AU - Bähler, J.

AU - Kohli, J.

AU - Hiraoka, Y.

PY - 2001

Y1 - 2001

N2 - Regular segregation of homologous chromosomes during meiotic divisions is essential for the generation of viable progeny. In recombination-proficient organisms, chromosome disjunction at meiosis I generally occurs by chiasma formation between the homologs (chiasmate meiosis). We have studied meiotic stages in living rec8 and rec7 mutant cells of fission yeast, with special attention to prophase and the first meiotic division. Both rec8 and rec7 are early recombination mutants, and in rec7 mutants, chromosome segregation at meiosis I occurs without any recombination (achiasmate meiosis). Both mutants showed distinct irregularities in nuclear prophase movements. Additionally, rec7 showed an extended first division of variable length and with single chromosomes changing back and forth between the cell poles. Two other early recombination deficient mutants (rec14 and rec15) showed very similar phenotypes to rec7 during the first meiotic division, and the fidelity of achiasmate chromosome segregation slightly exceeded the expected random level. We discuss possible regulatory mechanisms of fission yeast to deal with achiasmate chromosome segregation.

AB - Regular segregation of homologous chromosomes during meiotic divisions is essential for the generation of viable progeny. In recombination-proficient organisms, chromosome disjunction at meiosis I generally occurs by chiasma formation between the homologs (chiasmate meiosis). We have studied meiotic stages in living rec8 and rec7 mutant cells of fission yeast, with special attention to prophase and the first meiotic division. Both rec8 and rec7 are early recombination mutants, and in rec7 mutants, chromosome segregation at meiosis I occurs without any recombination (achiasmate meiosis). Both mutants showed distinct irregularities in nuclear prophase movements. Additionally, rec7 showed an extended first division of variable length and with single chromosomes changing back and forth between the cell poles. Two other early recombination deficient mutants (rec14 and rec15) showed very similar phenotypes to rec7 during the first meiotic division, and the fidelity of achiasmate chromosome segregation slightly exceeded the expected random level. We discuss possible regulatory mechanisms of fission yeast to deal with achiasmate chromosome segregation.

KW - Achiasmate segregation

KW - Chromosome segregation

KW - Fission yeast

KW - Meiosis

UR - http://www.scopus.com/inward/record.url?scp=0034871633&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034871633&partnerID=8YFLogxK

M3 - Article

VL - 114

SP - 2843

EP - 2853

JO - Journal of Cell Science

JF - Journal of Cell Science

SN - 0021-9533

IS - 15

ER -