Live imaging of centriole dynamics by fluorescently tagged proteins in starfish oocyte meiosis

Joana Borrego-Pinto, K. Somogyi, Péter Lénárt

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

High throughput DNA sequencing, the decreasing costs of DNA synthesis, and universal techniques for genetic manipulation have made it much easier and quicker to establish molecular tools for any organism than it has been 5 years ago. This opens a great opportunity for reviving “nonconventional” model organisms, which are particularly suited to study a specific biological process and many of which have already been established before the era of molecular biology. By taking advantage of transcriptomics, in particular, these systems can now be easily turned into full fetched models for molecular cell biology. As an example, here we describe how we established molecular tools in the starfish Patiria miniata, which has been a popular model for cell and developmental biology due to the synchronous and rapid development, transparency, and easy handling of oocytes, eggs, and embryos. Here, we detail how we used a de novo assembled transcriptome to produce molecular markers and established conditions for live imaging to investigate the molecular mechanisms underlying centriole elimination-a poorly understood process essential for sexual reproduction of animal species.

Original languageEnglish
Pages (from-to)145-166
Number of pages22
JournalMethods in Molecular Biology
Volume1457
DOIs
Publication statusPublished - 2016

Fingerprint

Starfish
Centrioles
Meiosis
Oocytes
Cell Biology
Molecular Biology
Biological Phenomena
Genetic Techniques
High-Throughput Nucleotide Sequencing
Developmental Biology
Transcriptome
Eggs
Reproduction
Proteins
Embryonic Structures
Costs and Cost Analysis
DNA

Keywords

  • Centriole elimination
  • Centrosome
  • Fluorescent markers
  • Live imaging
  • Meiosis
  • Microinjection
  • Starfish
  • Transcriptome

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Live imaging of centriole dynamics by fluorescently tagged proteins in starfish oocyte meiosis. / Borrego-Pinto, Joana; Somogyi, K.; Lénárt, Péter.

In: Methods in Molecular Biology, Vol. 1457, 2016, p. 145-166.

Research output: Contribution to journalArticle

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