Liquid phase separation methods for N-glycosylation analysis of glycoproteins of biomedical and biopharmaceutical interest. A critical review

Laszlo Hajba, Eszter Csanky, Andras Guttman

Research output: Contribution to journalReview article

21 Citations (Scopus)

Abstract

Comprehensive carbohydrate analysis of glycoproteins from human biological samples and biotherapeutics are important from diagnostic and therapeutic points of view. This review summarizes the current state-of-the-art liquid phase separation techniques used in N-glycosylation analysis. The different liquid chromatographic techniques and capillary electrophoresis methods are critically discussed in detail. Miniaturization of these methods is also important to increase throughput and decrease analysis time. The sample preparation and labeling methods for asparagine linked oligosaccharides are also addressed.

Original languageEnglish
Pages (from-to)8-16
Number of pages9
JournalAnalytica Chimica Acta
Volume943
DOIs
Publication statusPublished - Nov 2 2016

Keywords

  • Fluorescent labels
  • Liquid phase separations
  • N-glycans
  • Recombinant protein therapeutics
  • Serum glycoproteins

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy

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