Liquid chromatography/thermospray mass spectrometry of monosaccharides and their derivatives

Miklós Lipták, Z. Dinya, F. Sztaricskai, György Litkei, Jáozsef Jekö

Research output: Contribution to journalArticle

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Abstract

Thermospray (TSP) mass spectrometry has proved to be a useful technique for analysing various highly polar compounds. The use of a quadrupole mass spectrometer equipped with a thermospray/plasmaspray interface in the analysis of more than 30 monosaccharides and sugar derivatives is described. A 1:1 mixture of methanol or acetonitrile and 0.1 M aqueous ammonium acetate as eluent at 1 cm3 min−1 affords the best results. The correct setting of the capillary tip temperature and repeller voltage was fundamental for the ionization of carbohydrates. The/optimum values of these parameters were 240–250°C and 220–240 V, respectively. The thermospray mass spectra of most carbohydrates exhibit strong [M + NH4]+ ions which provide molecular mass information. The underivatized and derivatized monosaccharides could be grouped according to the presence or absence and the relative abundances of [M + NH4 − H2O]+, [M + H]+ and [M + NH4]+ ions.

Original languageEnglish
Pages (from-to)1271-1275
Number of pages5
JournalOrganic Mass Spectrometry
Volume27
Issue number11
DOIs
Publication statusPublished - 1992

Fingerprint

monosaccharides
Monosaccharides
Liquid chromatography
carbohydrates
liquid chromatography
Carbohydrates
Liquid Chromatography
Mass spectrometry
Mass Spectrometry
mass spectroscopy
Ions
Derivatives
Molecular mass
Mass spectrometers
sugars
Acetonitrile
Sugars
mass spectrometers
mass spectra
acetonitrile

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Instrumentation

Cite this

Liquid chromatography/thermospray mass spectrometry of monosaccharides and their derivatives. / Lipták, Miklós; Dinya, Z.; Sztaricskai, F.; Litkei, György; Jekö, Jáozsef.

In: Organic Mass Spectrometry, Vol. 27, No. 11, 1992, p. 1271-1275.

Research output: Contribution to journalArticle

Lipták, Miklós ; Dinya, Z. ; Sztaricskai, F. ; Litkei, György ; Jekö, Jáozsef. / Liquid chromatography/thermospray mass spectrometry of monosaccharides and their derivatives. In: Organic Mass Spectrometry. 1992 ; Vol. 27, No. 11. pp. 1271-1275.
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