Liquid chromatographic monitoring of phytoecdysteroid production of Serratula wolffii

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Abstract

Seasonal dependence of 20-hydroxyecdysone content of Serratula wolffii was monitored. Thin-layer chromatography was used after a single clean-up using polyamide. High performance liquid chromatography was employed for the analysis, after removal of the high excess of flavonoids and other impurities using a clean-up on both C18 and polyamide. Parallel analyses using both thin layer chromatography with densitometry and high performance liquid chromatography gave similar results at the determination of vegetation dependence of ecdysteroids. Results indicated two maxima of ecdysteroid content (w/w), such as beginning of vegetation (in April and May) and during blossoming (in August). The two ecdysteroids, 20-hydroxyecdysone and polypodine B were also isolated from Serratula wolffii, their chemical structures were identified by spectroscopic methods.

Original languageEnglish
Pages (from-to)281-294
Number of pages14
JournalJournal of Liquid Chromatography and Related Technologies
Volume23
Issue number2
DOIs
Publication statusPublished - 2000

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Ecdysteroids
Ecdysterone
Thin layer chromatography
Nylons
High performance liquid chromatography
Thin Layer Chromatography
Monitoring
Liquids
High Pressure Liquid Chromatography
Densitometry
Flavonoids
Impurities

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Analytical Chemistry

Cite this

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abstract = "Seasonal dependence of 20-hydroxyecdysone content of Serratula wolffii was monitored. Thin-layer chromatography was used after a single clean-up using polyamide. High performance liquid chromatography was employed for the analysis, after removal of the high excess of flavonoids and other impurities using a clean-up on both C18 and polyamide. Parallel analyses using both thin layer chromatography with densitometry and high performance liquid chromatography gave similar results at the determination of vegetation dependence of ecdysteroids. Results indicated two maxima of ecdysteroid content (w/w), such as beginning of vegetation (in April and May) and during blossoming (in August). The two ecdysteroids, 20-hydroxyecdysone and polypodine B were also isolated from Serratula wolffii, their chemical structures were identified by spectroscopic methods.",
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T1 - Liquid chromatographic monitoring of phytoecdysteroid production of Serratula wolffii

AU - Báthori, M.

AU - Gergely, A.

AU - Kalász, H.

AU - Nagy, G.

AU - Dobos, Á

AU - Máthé, I.

PY - 2000

Y1 - 2000

N2 - Seasonal dependence of 20-hydroxyecdysone content of Serratula wolffii was monitored. Thin-layer chromatography was used after a single clean-up using polyamide. High performance liquid chromatography was employed for the analysis, after removal of the high excess of flavonoids and other impurities using a clean-up on both C18 and polyamide. Parallel analyses using both thin layer chromatography with densitometry and high performance liquid chromatography gave similar results at the determination of vegetation dependence of ecdysteroids. Results indicated two maxima of ecdysteroid content (w/w), such as beginning of vegetation (in April and May) and during blossoming (in August). The two ecdysteroids, 20-hydroxyecdysone and polypodine B were also isolated from Serratula wolffii, their chemical structures were identified by spectroscopic methods.

AB - Seasonal dependence of 20-hydroxyecdysone content of Serratula wolffii was monitored. Thin-layer chromatography was used after a single clean-up using polyamide. High performance liquid chromatography was employed for the analysis, after removal of the high excess of flavonoids and other impurities using a clean-up on both C18 and polyamide. Parallel analyses using both thin layer chromatography with densitometry and high performance liquid chromatography gave similar results at the determination of vegetation dependence of ecdysteroids. Results indicated two maxima of ecdysteroid content (w/w), such as beginning of vegetation (in April and May) and during blossoming (in August). The two ecdysteroids, 20-hydroxyecdysone and polypodine B were also isolated from Serratula wolffii, their chemical structures were identified by spectroscopic methods.

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