Liquid chromatographic method for the determination of ticlopidine in human plasma

K. Róna, K. Ary, B. Gachályi, I. Klebovich

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

A simple high-performance liquid chromatographic method for determination of ticlopidine in human plasma using ultra violet detection was developed. The separation of the investigated compound and internal standard was achieved on a C,, BD column with a 0.01 M potassium dihydrogen phosphate buffer (pH 4)-acetonitrile-methanol (20:40:40, v/v) mobile phase. The detection was performed at 215 nm. The compounds were isolated from plasma by Bond Elut C18 solid-phase extraction, the mean absolute recovery was 84.9%. The limit of quantitation was 10 ng ml-1, the limit of detection was 5 ng ml-1. The bioanalytical method was validated with respect to linearity, within- and between-day accuracy and precision, system suitability and stability. All validated parameters were found to be within the internationally required limits. The developed analytical method for ticlopidine was found to be suitable for application in pharmacokinetic studies and human drug monitoring.

Original languageEnglish
Pages (from-to)393-398
Number of pages6
JournalJournal of Chromatography B: Biomedical Applications
Volume693
Issue number2
DOIs
Publication statusPublished - Jun 6 1997

Fingerprint

Plasma (human)
Ticlopidine
Pharmacokinetics
Liquids
Methanol
Buffers
Plasmas
Recovery
Monitoring
Pharmaceutical Preparations
potassium phosphate
acetonitrile

Keywords

  • Ticlopidine

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Liquid chromatographic method for the determination of ticlopidine in human plasma. / Róna, K.; Ary, K.; Gachályi, B.; Klebovich, I.

In: Journal of Chromatography B: Biomedical Applications, Vol. 693, No. 2, 06.06.1997, p. 393-398.

Research output: Contribution to journalArticle

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