Liquid chromatographic enantioseparation of β-blocking agents with (1R,2R)-1,3-diacetoxy-1-(4-nitrophenyl)-2-propyl isothiocyanate as chiral derivatizing agent

M. Péter, Á Gyéresi, F. Fülöp

Research output: Contribution to journalArticle

27 Citations (Scopus)


The applicability of (1R,2R)-1,3-diacetoxy-1-(4-nitrophenyl)-2-propyl isothiocyanate [(R,R)-DANI] as a recently developed chiral derivatizing agent for the enantioseparation of a series of β-blockers is described. The thiourea diastereomers formed were analyzed by reversed-phase high-performance liquid chromatography, mixtures of water and methanol or acetonitrile being used for elution. Conditions of derivatizations (temperature, reagent excess and reaction time) were optimized, and the effects of organic modifiers on the retention and separation were investigated; the diastereomers could readily be baseline separated with methanol-containing mobile phases with resolutions between 1.58 and 2.72.

Original languageEnglish
Pages (from-to)247-253
Number of pages7
JournalJournal of Chromatography A
Issue number2
Publication statusPublished - Mar 2 2001



  • (1R,2R)-1,3-diacetoxy-1-(4-nitrophenyl )-2-propyl isothiocyanate
  • Derivatization, LC
  • Enantiomer separation
  • β-Blockers

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

Cite this