Liquid chromatographic determination of the antibiotic fumagillin in fish meat samples

J. Fekete, Zsuzsanna Romvári, Ildikó Szepesi, György Morovján

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

A procedure for the determination of fumagillin, an antibiotic of Aspergillus fumigatus in fish meat samples, using reversed-phase high-performance liquid chromatography is described. Liquid chromatography was performed on an octadecylsilane column using acetonitrile-water-phosphoric acid solution as mobile phase, with detection at 350 nm. Two different types of sample preparation were developed, clean-up and enrichment, and the limits of quantification were 100 ng/g and 5 ng/g, respectively, in fish meat. The recovery was 75±3% in the concentration range 100-500 ng/g. To introduce the methodology and demonstrate its usefulness, a practical experiment was performed. Trouts fed with fumagillin were examined for elimination of fumagillin. After 24 h, the concentration was shown to decrease to below 100 ng/g.

Original languageEnglish
Pages (from-to)378-381
Number of pages4
JournalJournal of Chromatography A
Volume712
Issue number2
DOIs
Publication statusPublished - Oct 13 1995

Fingerprint

Meats
Meat
Fish
Fishes
Anti-Bacterial Agents
Liquids
Aspergillus fumigatus
Trout
Aspergillus
Liquid chromatography
High performance liquid chromatography
Reverse-Phase Chromatography
Liquid Chromatography
High Pressure Liquid Chromatography
Recovery
Water
fumagillin
Experiments

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Organic Chemistry

Cite this

Liquid chromatographic determination of the antibiotic fumagillin in fish meat samples. / Fekete, J.; Romvári, Zsuzsanna; Szepesi, Ildikó; Morovján, György.

In: Journal of Chromatography A, Vol. 712, No. 2, 13.10.1995, p. 378-381.

Research output: Contribution to journalArticle

Fekete, J. ; Romvári, Zsuzsanna ; Szepesi, Ildikó ; Morovján, György. / Liquid chromatographic determination of the antibiotic fumagillin in fish meat samples. In: Journal of Chromatography A. 1995 ; Vol. 712, No. 2. pp. 378-381.
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