Lipoxygenase and glutathione peroxidase activity in tobacco leaves inoculated with tobacco mosaic virus

A. Künstler, L. Király, M. Pogány, I. Tóbiás, G. Gullner

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Fatty acid hydroperoxide-producing lipoxygenase (LOX) and hydroperoxide-degrading glutathione peroxidase (GPOX) enzyme activities were studied in leaves of virus resistant Xanthi-nc and susceptible Samsun-nn tobacco cultivars after inoculation with Tobacco mosaic virus (TMV). Total LOX activity showed a maximum at pH 5.5 in cell-free extracts of uninfected leaves. LOX activity markedly increased at this pH after TMV inoculation, but a substantial induction was detected also in the basic pH range with an emerging peak around pH = 8.5. TMV-elicited LOX induction was weaker and appeared later in Samsun-nn than in Xanthi-nc leaves. GPOX activity was also substantially induced by TMV infection. However, this induction appeared only 4 days post-inoculation in resistant Xanthi-nc plants in tissues surrounding the localized necrotic lesions. In contrast, GPOX activity did not change in TMV-inoculated, susceptible Samsun-nn leaves. Several glutathione S-transferase (GST) isoenzymes also display GPOX activity. The expression of a tau class GST gene was markedly induced by TMV inoculation in Xanthi-nc leaves. This tobacco GST gene was partially cloned and sequenced.

Original languageEnglish
Pages (from-to)197-207
Number of pages11
JournalActa Phytopathologica et Entomologica Hungarica
Volume42
Issue number2
DOIs
Publication statusPublished - Dec 2007

Fingerprint

Tobacco mosaic virus
lipoxygenase
glutathione peroxidase
tobacco
glutathione transferase
leaves
phospholipid-hydroperoxide glutathione peroxidase
lesions (plant)
hydroperoxides
leaf extracts
plant tissues
isozymes
genes
enzyme activity
fatty acids
viruses
cultivars
infection
cells

Keywords

  • Fatty acid hydroperoxide
  • Glutathione peroxidase
  • Glutathione S-transferase
  • Hypersensitive reaction
  • Lipid peroxidation
  • Lipoxygenase
  • Tobacco
  • Tobacco mosaic virus

ASJC Scopus subject areas

  • Insect Science
  • Plant Science

Cite this

Lipoxygenase and glutathione peroxidase activity in tobacco leaves inoculated with tobacco mosaic virus. / Künstler, A.; Király, L.; Pogány, M.; Tóbiás, I.; Gullner, G.

In: Acta Phytopathologica et Entomologica Hungarica, Vol. 42, No. 2, 12.2007, p. 197-207.

Research output: Contribution to journalArticle

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