Liposome-mediated gene transfer in fish embryos

József Szelei, László Váradi, Ferenc Müller, F. Erdélyi, László Orbán, László Horváth, E. Duda

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Liposome-mediated gene transfer was used to introduce large DNA constructs into zygotes of African catfish. The technique is based on the delivery of recombinant bacteriophage lambda particles (or DNA-protamine complexes) into the cytoplasm of target cells by negatively charged, large unilamellar liposomes. Dechorionated zygotes and early fish embryos were treated with the transforming liposomes. Expression of the introduced reporter genes during the first three weeks of the development of the larvae was followed by measuring the activity of corresponding enzymes. These assays have indicated very efficient DNA uptake into the embryos.

Original languageEnglish
Pages (from-to)116-119
Number of pages4
JournalTransgenic Research
Volume3
Issue number2
DOIs
Publication statusPublished - Mar 1994

Fingerprint

lipid bodies
gene transfer
Liposomes
embryo (animal)
Fishes
Embryonic Structures
Zygote
zygote
DNA
fish
Genes
protamines
Bacteriophage lambda
Catfishes
Unilamellar Liposomes
Protamines
Reporter Genes
reporter genes
catfish
bacteriophages

Keywords

  • bacteriophage lambda
  • reporter gene
  • transient expression
  • zygote

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Genetics
  • Biotechnology
  • Plant Science
  • Food Science

Cite this

Szelei, J., Váradi, L., Müller, F., Erdélyi, F., Orbán, L., Horváth, L., & Duda, E. (1994). Liposome-mediated gene transfer in fish embryos. Transgenic Research, 3(2), 116-119. https://doi.org/10.1007/BF01974089

Liposome-mediated gene transfer in fish embryos. / Szelei, József; Váradi, László; Müller, Ferenc; Erdélyi, F.; Orbán, László; Horváth, László; Duda, E.

In: Transgenic Research, Vol. 3, No. 2, 03.1994, p. 116-119.

Research output: Contribution to journalArticle

Szelei, J, Váradi, L, Müller, F, Erdélyi, F, Orbán, L, Horváth, L & Duda, E 1994, 'Liposome-mediated gene transfer in fish embryos', Transgenic Research, vol. 3, no. 2, pp. 116-119. https://doi.org/10.1007/BF01974089
Szelei J, Váradi L, Müller F, Erdélyi F, Orbán L, Horváth L et al. Liposome-mediated gene transfer in fish embryos. Transgenic Research. 1994 Mar;3(2):116-119. https://doi.org/10.1007/BF01974089
Szelei, József ; Váradi, László ; Müller, Ferenc ; Erdélyi, F. ; Orbán, László ; Horváth, László ; Duda, E. / Liposome-mediated gene transfer in fish embryos. In: Transgenic Research. 1994 ; Vol. 3, No. 2. pp. 116-119.
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