Liposome flow injection immunoassay: Model calculations of competitive immunoreactions involving univalent and multivalent ligands

William T. Yap, Laurie Locascio-Brown, Anne L. Plant, Steven J. Choquette, V. Horváth, Richard A. Durst

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

The use of liposomes as detectable reagents in solid-phase immunoassays has been explored in a flow injection immunoanalysis (FIIA) system. Model calculations are presented for FIIA based on the competitive binding of univalent analyte and multivalent liposomes to immobilized antibodies. Parameters such as binding constants, concentrations of liposomes and antibody, and steric hindrance are considered for their relative effects on detectable liposome signal response to analyte concentrations. Qualitative comparisons of the model with the experimental data are made.

Original languageEnglish
Pages (from-to)2007-2011
Number of pages5
JournalAnalytical Chemistry
Volume63
Issue number18
Publication statusPublished - Sep 15 1991

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Liposomes
Ligands
Immobilized Antibodies
Antibodies

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Yap, W. T., Locascio-Brown, L., Plant, A. L., Choquette, S. J., Horváth, V., & Durst, R. A. (1991). Liposome flow injection immunoassay: Model calculations of competitive immunoreactions involving univalent and multivalent ligands. Analytical Chemistry, 63(18), 2007-2011.

Liposome flow injection immunoassay : Model calculations of competitive immunoreactions involving univalent and multivalent ligands. / Yap, William T.; Locascio-Brown, Laurie; Plant, Anne L.; Choquette, Steven J.; Horváth, V.; Durst, Richard A.

In: Analytical Chemistry, Vol. 63, No. 18, 15.09.1991, p. 2007-2011.

Research output: Contribution to journalArticle

Yap, William T. ; Locascio-Brown, Laurie ; Plant, Anne L. ; Choquette, Steven J. ; Horváth, V. ; Durst, Richard A. / Liposome flow injection immunoassay : Model calculations of competitive immunoreactions involving univalent and multivalent ligands. In: Analytical Chemistry. 1991 ; Vol. 63, No. 18. pp. 2007-2011.
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