Lipopolysaccharide exposure makes allergic airway inflammation and hyperresponsiveness less responsive to dexamethasone and inhibition of iNOS

Z. I. Komlósi, É Pozsonyi, T. Tábi, É Szöko, A. Nagy, B. Bartos, G. T. Kozma, L. Tamási, M. Orosz, P. Magyar, G. Losonczy

Research output: Contribution to journalArticle

20 Citations (Scopus)


Allergic airway disease can be refractory to anti-inflammatory treatment, whose cause is unclarified. Therefore, in the present experiment, we have tested the hypothesis that co-exposure to lipopolysacharide (Lps) and allergen results in glucocorticoid-resistant eosinophil airway inflammation and hyper-responsiveness (AHR). Ovalbumin (Ova)-sensitized BALB/c mice were primed with 10 μg intranasal Lps 24 h before the start of Ova challenges (20 min on 3 consecutive days). Dexamethasone (5 mg/kg/day) was given on the last 2 days of Ova challenges. AHR, cellular build-up, cytokine and nitrite concentrations of bronchoalveolar lavage fluid (BALF) and lung histology were examined. To assess the role of iNOS-derived NO in airway responsiveness, mice were treated with a selective inhibitor of this enzyme (1400W) 2 h before AHR measurements. More severe eosinophil inflammation and higher nitrite formation were found in Lps-primed than in non-primed allergized mice. After Lps priming, AHR and concentrations of T-helper type 2 cytokines in BALF were decreased, but still remained significantly higher than in controls. Eosinophil inflammation was partially, while nitrite production and AHR were observed to be largely dexamethasone resistant in Lps-primed allergized animals. 1400W effectively and rapidly diminished the AHR in Ova-sensitized and challenged mice, but failed to affect it after Lps priming plus allergization. In conclusion, Lps inhalation may exaggerate eosinophil inflammation and reduce responsiveness to anti-inflammatory treatment in allergic airway disease.

Original languageEnglish
Pages (from-to)951-959
Number of pages9
JournalClinical and Experimental Allergy
Issue number7
Publication statusPublished - Jul 1 2006


  • 1400W
  • Asthma
  • Eosinophil airway inflammation
  • Hyper-responsiveness
  • Lipopolysaccharide
  • Nitric oxide
  • Ovalbumin
  • Steroid

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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