Lipophilicity determination of some photosynthetic pigments by reversed-phase thin-layer chromatography. The effect of support and the organic phase in the eluent

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Abstract

The lipophilicity of some photosynthetic pigments was determined by reversed-phase thin-layer chromatography using silica, alumina, cellulose, polyamid and diatomaceous earth as supports and acetone and ethanol as organic mobile phase. The RM value of each compound linearly decreased with increasing concentration of the organic modifier. The supports exerted high impact on the separation: no acceptable separation was achieved on cellulose, polyamid, alumina and diatomaceous earth supports while silica supports produced the best separation. The retention of photosynthetic pigments depended on the origin of silica and on the type of organic mobile phase.

Original languageEnglish
Pages (from-to)908-914
Number of pages7
JournalChromatographia
Volume25
Issue number10
DOIs
Publication statusPublished - Oct 1 1988

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Keywords

  • Photosynthetic pigments
  • Supports for reversed-phase TLC
  • Thin-layer chromtography

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Organic Chemistry

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